Wise, Helen M., Foeglein, Agnes, Sun, Jiechao, Dalton, Rosa Maria, Patel, Sheetal, Howard, Wendy, Anderson, Emma C., Barclay, Wendy S. and Digard, Paul. (2009) A complicated message: identification of a novel PB1-related protein translated from influenza A virus segment 2 mRNA. Journal of Virology, Vol.83 (No.16). pp. 8021-8031. ISSN 0022-538X

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Abstract

Influenza A virus segment 2 is known to encode two polypeptides in overlapping open reading frames: PB1, the polymerase, and PB1-F2, a proapoptotic virulence factor. We show that a third major polypeptide is synthesized from PB1 mRNA via differential AUG codon usage. PB1 codon 40 directs translation of an N-terminally truncated version of the polypeptide (N40) that lacks transcriptase function but nevertheless interacts with PB2 and the polymerase complex in the cellular environment. Importantly, the expression of N40, PB1-F2, and PB1 are interdependent, and certain mutations previously used to ablate PB1-F2 production affected N40 accumulation. Removal of the PB1-F2 AUG upregulated N40 synthesis, while truncating PB1-F2 after codon 8 ( with a concomitant M40I change in PB1) abolished N40 expression. A virus lacking both N40 and PB1-F2 replicated normally. However, viruses that did not express N40 but retained an intact PB1-F2 gene overexpressed PB1 early in infection and replicated slowly in tissue culture. Thus, the influenza A virus proteome includes a 12th primary translation product that (similarly to PB1-F2) is nonessential for virus viability but whose loss, in particular genetic backgrounds, is detrimental to virus replication.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Journal of Virology
Publisher:	American Society for Microbiology
ISSN:	0022-538X
Official Date:	15 August 2009
Volume:	Vol.83
Number:	No.16
Number of Pages:	11
Page Range:	pp. 8021-8031
Identification Number:	10.1128/JVI.00826-09
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Medical Research Council, Wellcome Trust
Grant number:	G0700815
URI:	http://wrap.warwick.ac.uk/id/eprint/27589

Data sourced from Thomson Reuters' Web of Knowledge

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