A comparison of labeling and label-free mass spectrometry-based proteomics approaches
Patel, Vibhuti J., Thalassinos, Konstantinos, Slade, Susan E., Connolly, Joanne B., Crombie, Andrew, Murrell, J. C. (J. Colin) and Scrivens, James H.. (2009) A comparison of labeling and label-free mass spectrometry-based proteomics approaches. Journal of Proteome Research, Vol.8 (No.7). pp. 3752-3759. ISSN 1535-3893Full text not available from this repository.
Official URL: http://dx.doi.org/10.1021/pr900080y
The proteome of the recently discovered bacterium Methyiocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Journal of Proteome Research|
|Publisher:||American Chemical Society|
|Number of Pages:||8|
|Page Range:||pp. 3752-3759|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Biological Mass Spectrometry and Protcomics Facility, Department of Biological Sciences, Warwick|
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