Patel, Vibhuti J., Thalassinos, Konstantinos, Slade, Susan E., Connolly, Joanne B., Crombie, Andrew, Murrell, J. C. (J. Colin) and Scrivens, James H.. (2009) A comparison of labeling and label-free mass spectrometry-based proteomics approaches. Journal of Proteome Research, Vol.8 (No.7). pp. 3752-3759. ISSN 1535-3893

Full text not available from this repository.

Abstract

The proteome of the recently discovered bacterium Methyiocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Journal of Proteome Research
Publisher:	American Chemical Society
ISSN:	1535-3893
Date:	July 2009
Volume:	Vol.8
Number:	No.7
Number of Pages:	8
Page Range:	pp. 3752-3759
Identification Number:	10.1021/pr900080y
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biological Mass Spectrometry and Protcomics Facility, Department of Biological Sciences, Warwick
URI:	http://wrap.warwick.ac.uk/id/eprint/27590

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item