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Mass spectrometric study of glucose and cellobiose produced during enzymatic hydrolysis of alpha-cellulose extracted from oak late-wood annual rings

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Sensula, Barbara M., Derrick, Peter J. , Bickerton, John and Pazdur, Anna (2009) Mass spectrometric study of glucose and cellobiose produced during enzymatic hydrolysis of alpha-cellulose extracted from oak late-wood annual rings. Rapid Communications in Mass Spectrometry, Vol.23 (No.13). pp. 2070-2074. doi:10.1002/rcm.4089

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Official URL: http://dx.doi.org/10.1002/rcm.4089

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Abstract

We present the first results concerning interannual variations in concentrations of glucose and cellobiose, obtained through enzymatic hydrolysis of alpha-cellulose. The alpha-cellulose was extracted from late-wood of oak. The tree-ring chronologies, wood components and their physical and chemical properties provide information about the ecosystem in which the tree grew, and thus information regarding climate variability and the impact of human activity in the past. The large molecular size and insolubility make it difficult to determine precisely the chemical and physical properties of the intact cellulose polymer. Enzymatic hydrolysis is the principal method of degradation of cellulose. In this study the feasibility has been examined of characterizing (alpha-cellulose through analysis by mass spectrometry (MS) of the degradation products from hydrolysis. Degradation of alpha-cellulose was possible without using alkaline or acid buffers. Analysis by MS provided the opportunity to obtain information on the biodegradation of saccharides. The presence of cellobiose and glucose in the degradation product was evidenced by the mass spectra. We have compared the abundances of these glucose and cellobiose ions with carbon isotope ratios, the efficiency of extraction of alpha-cellulose from the wood and tree-ring width indices. The challenge is to establish, with respect to climate changes and environmental conditions, the significance of the variations from one year to another in the observed abundances of glucose and cellobiose ions. Copyright (C) 2009 John Wiley & Sons, Ltd.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QC Physics
Divisions: Faculty of Science > Chemistry
Journal or Publication Title: Rapid Communications in Mass Spectrometry
Publisher: John Wiley & Sons Ltd.
ISSN: 0951-4198
Official Date: July 2009
Dates:
DateEvent
July 2009Published
Volume: Vol.23
Number: No.13
Number of Pages: 5
Page Range: pp. 2070-2074
DOI: 10.1002/rcm.4089
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Marie Curie Fellowship Scheme, British Council Young Scientists Programme

Data sourced from Thomson Reuters' Web of Knowledge

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