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Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer
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Knowles, Tim, Finka, Rachael, Smith, Corinne J., Lin, Yu-Pin, Dafforn, Tim and Overduin, Michael (2009) Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer. Journal of the American Chemical Society, Vol.131 (No.22). pp. 7484-7485. doi:10.1021/ja810046q ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja810046q
Abstract
One-third of eukaryotic proteins are integrated within membranes, as are the targets of 40% of approved drugs. However, the lack of a general means of solubilizing, stabilizing and structurally characterizing active membrane proteins has frustrated efforts to understand their mechanisms and exploit their potential value. Here we report that bilayer disks formed by phospholipids and styrene maleic anhydride copolymer preserve the functional and structural integrity of alpha-helical and beta-barrel transmembrane proteins. They form 11 nm particles that are monodispersed, biocompatible, thermostable, and water-soluble, allowing diverse membrane proteins to be simply and rapidly presented for virtually any in vitro analysis.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
Journal or Publication Title: | Journal of the American Chemical Society | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 0002-7863 | ||||
Official Date: | 10 June 2009 | ||||
Dates: |
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Volume: | Vol.131 | ||||
Number: | No.22 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 7484-7485 | ||||
DOI: | 10.1021/ja810046q | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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