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A microelectrode biosensor for real time monitoring of L-glutamate release

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Tian, Faming, Gourine, Alexander V., Huckstepp, Robert T. R. and Dale, Nicholas (2009) A microelectrode biosensor for real time monitoring of L-glutamate release. Analytica Chimica Acta, Vol.645 (No.1-2). pp. 86-91. doi:10.1016/j.aca.2009.04.048

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Official URL: http://dx.doi.org/10.1016/j.aca.2009.04.048

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Abstract

We have developed an amperometric microbiosensor for real time monitoring L-glutamate release in neural tissue, based on enzymatic oxidation catalyzed by the L-glutamate oxidase. By means of a sol-gel coating method, L-glutamate oxidase was entrapped in a biocompatible gel layer that provided a benign environment and retained enzyme activity on the surface of Pt microelectrode. Prior to gel layer formation, a modification on the surface of Pt microelectrode with poly(phenylene diamine) enabled the microbiosensor screen majority of common potential interfering substances existing in physiological samples. The miniaturized biosensor achieved a steady state response to L-glutamate within 10s and exhibited a linear dependence on the concentration of L-glutamate from 0.5 to 100 mu mol L-1 with a high sensitivity of 279.4 +/- 2.0 mu A(mmol L-1)(-1) cm(-2) (n=4, R.S.D. = 2.8%). The microbiosensor also exhibited excellent long-term stability in dry storage. We have successfully used the microbiosensor for real time measuring of L-glutamate in vivo. (C) 2009 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title: Analytica Chimica Acta
Publisher: Elsevier Science BV
ISSN: 0003-2670
Official Date: 10 July 2009
Dates:
DateEvent
10 July 2009Published
Volume: Vol.645
Number: No.1-2
Number of Pages: 6
Page Range: pp. 86-91
DOI: 10.1016/j.aca.2009.04.048
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

Data sourced from Thomson Reuters' Web of Knowledge

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