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A microelectrode biosensor for real time monitoring of L-glutamate release
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Tian, Faming, Gourine, Alexander V., Huckstepp, Robert T. R. and Dale, Nicholas (2009) A microelectrode biosensor for real time monitoring of L-glutamate release. Analytica Chimica Acta, Vol.645 (No.1-2). pp. 86-91. doi:10.1016/j.aca.2009.04.048
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Official URL: http://dx.doi.org/10.1016/j.aca.2009.04.048
Abstract
We have developed an amperometric microbiosensor for real time monitoring L-glutamate release in neural tissue, based on enzymatic oxidation catalyzed by the L-glutamate oxidase. By means of a sol-gel coating method, L-glutamate oxidase was entrapped in a biocompatible gel layer that provided a benign environment and retained enzyme activity on the surface of Pt microelectrode. Prior to gel layer formation, a modification on the surface of Pt microelectrode with poly(phenylene diamine) enabled the microbiosensor screen majority of common potential interfering substances existing in physiological samples. The miniaturized biosensor achieved a steady state response to L-glutamate within 10s and exhibited a linear dependence on the concentration of L-glutamate from 0.5 to 100 mu mol L-1 with a high sensitivity of 279.4 +/- 2.0 mu A(mmol L-1)(-1) cm(-2) (n=4, R.S.D. = 2.8%). The microbiosensor also exhibited excellent long-term stability in dry storage. We have successfully used the microbiosensor for real time measuring of L-glutamate in vivo. (C) 2009 Elsevier B.V. All rights reserved.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | Analytica Chimica Acta | ||||
| Publisher: | Elsevier Science BV | ||||
| ISSN: | 0003-2670 | ||||
| Official Date: | 10 July 2009 | ||||
| Dates: |
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| Volume: | Vol.645 | ||||
| Number: | No.1-2 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 86-91 | ||||
| DOI: | 10.1016/j.aca.2009.04.048 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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