Tian, Faming, Gourine, Alexander V., Huckstepp, Robert T. R. and Dale, Nicholas. (2009) A microelectrode biosensor for real time monitoring of L-glutamate release. Analytica Chimica Acta, Vol.645 (No.1-2). pp. 86-91. ISSN 0003-2670

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Abstract

We have developed an amperometric microbiosensor for real time monitoring L-glutamate release in neural tissue, based on enzymatic oxidation catalyzed by the L-glutamate oxidase. By means of a sol-gel coating method, L-glutamate oxidase was entrapped in a biocompatible gel layer that provided a benign environment and retained enzyme activity on the surface of Pt microelectrode. Prior to gel layer formation, a modification on the surface of Pt microelectrode with poly(phenylene diamine) enabled the microbiosensor screen majority of common potential interfering substances existing in physiological samples. The miniaturized biosensor achieved a steady state response to L-glutamate within 10s and exhibited a linear dependence on the concentration of L-glutamate from 0.5 to 100 mu mol L-1 with a high sensitivity of 279.4 +/- 2.0 mu A(mmol L-1)(-1) cm(-2) (n=4, R.S.D. = 2.8%). The microbiosensor also exhibited excellent long-term stability in dry storage. We have successfully used the microbiosensor for real time measuring of L-glutamate in vivo. (C) 2009 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Analytica Chimica Acta
Publisher:	Elsevier Science BV
ISSN:	0003-2670
Official Date:	10 July 2009
Volume:	Vol.645
Number:	No.1-2
Number of Pages:	6
Page Range:	pp. 86-91
Identification Number:	10.1016/j.aca.2009.04.048
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/27813

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