Antimicrobial activity of Coniothyrium minitans and its macrolide antibiotic macrosphelide A
Tomprefa, N., McQuilken, M. P., Hill, R. A. and Whipps, J. M.. (2009) Antimicrobial activity of Coniothyrium minitans and its macrolide antibiotic macrosphelide A. Journal of Applied Microbiology, Vol.106 (No.6). pp. 2048-2056. ISSN 1364-5072Full text not available from this repository.
Official URL: http://dx.doi.org/10.1111/j.1365-2672.2009.04174.x
Aims: Assessment of antimicrobial activity of the mycoparasite Coniothyrium minitans and its macrolide antibiotic macrosphelide A.
Methods and Results: Thirteen isolates of C minitans were tested for ability to inhibit a number of filamentous fungi, yeasts, oomycetes and bacteria in agar based tests. Activity was found against some ascomycetes, basidiomycetes, oomycetes and Gram-positive bacteria, but not against zygomycetes, yeasts or Gram-negative bacteria tested. Six C. minitans isolates (Conio, Contans, IVT1, CM/AP/3118, B279/1, Al/327/1) were found to produce macrosphelide A in liquid culture and no other antibiotics were detected. On agar, macrosphelide A inhibited growth of some ascomycetes, basidiomycetes, oomycetes and all four Gram-positive bacteria tested, including the medically important Staphylococcus aureus with a minimum inhibitory concentration of <= 500 mu g ml(-1). There was no inhibition observed against the yeasts and Gram-negative bacteria when macrosphelide A was tested at 700 mu g ml(-1).
Conclusions: The spectrum and level of activity of macrosphelide A produced by C. minitans against micro-organisms are extended markedly compared to previous reports.
Significance and Impact of the Study: Macrosphelide A was effective against Staph. aureus. Further study on the control of this bacterium is merited in view of the development of antibiotic resistance.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology
|Journal or Publication Title:||Journal of Applied Microbiology|
|Publisher:||Wiley-Blackwell Publishing Ltd.|
|Official Date:||June 2009|
|Number of Pages:||9|
|Page Range:||pp. 2048-2056|
|Funder:||Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)|
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