Han, Bing, Su, Tao, Wu, Hao, Gou, Zhongxuan, Xing, Xin-Hui, Jiang, Hao, Chen, Yin, Li, Xin and Murrell, J. C. (J. Colin). (2009) Paraffin oil as a "methane vector" for rapid and high cell density cultivation of Methylosinus trichosporium OB3b. Applied Microbiology and Biotechnology, Vol.83 (No.4). pp. 669-677. ISSN 0175-7598

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Abstract

Slow growth and relatively low cell densities of methanotrophs have limited their uses in industrial applications. In this study, a novel method for rapid cultivation of Methylosinus trichosporium OB3b was studied by adding a water-immiscible organic solvent in the medium. Paraffin oil was the most effective at enhancing cell growth and final cell density. This is at least partially due to the increase of methane gas transfer between gas and medium phases since methane solubility is higher in paraffin than in water/nitrate minimal salt medium. During cultivation with paraffin oil at 5% (v/v) in the medium, M. trichosporium OB3b cells also showed higher concentrations of the intermediary metabolites, such as formic acid and pyruvic acid, and consumed more methane compared with the control. Paraffin as methane vector to improve methanotroph growth was further studied in a 5-L fermentor at three concentrations (i.e., 2.5%, 5%, and 10%). Cell density reached about 14 g dry weight per liter with 5% paraffin, around seven times higher than that of the control (without paraffin). Cells cultivated with paraffin tended to accumulate around the interface between oil droplets and the water phase and could exist in oil phase in the case of 10% (v/v) paraffin. These results indicated that paraffin could enhance methanotroph growth, which is potentially useful in cultivation of methanotrophs in large scale in industry.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Applied Microbiology and Biotechnology
Publisher:	Springer
ISSN:	0175-7598
Date:	June 2009
Volume:	Vol.83
Number:	No.4
Number of Pages:	9
Page Range:	pp. 669-677
Identification Number:	10.1007/s00253-009-1866-2
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Ministry of Science and Technology of China, National Natural Science foundation of China, PetroChina Foundation
Grant number:	2006AA02Z203, 2033010, 050511-4-4
URI:	http://wrap.warwick.ac.uk/id/eprint/27873

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