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Apparent non-statistical binding in a ditopic receptor for guanosine

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Likhitsup, Asawin, Deeth, Robert J., Otto, Sijbren and Marsh, Andrew. (2009) Apparent non-statistical binding in a ditopic receptor for guanosine. Organic & Biomolecular Chemistry, Vol.7 (No.10). pp. 2093-2103. ISSN 1477-0520

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Official URL: http://dx.doi.org/10.1039/b812969j

Abstract

Analysis of stepwise association constants for guests binding to more than one site in a receptor is expected to give a ratio of the first association constant to the second of about 4 : 1 on statistical grounds (since a second guest should have an equal chance of binding to a different site on the same, or a new molecule). Taking account of self-association in our analysis of a system in which the binding sites are close together, we observe a ratio closer to 1 : 1, indicative of non-statistical, or cooperative binding. The longer homologue built around two alkynes displays a very different ratio of stepwise association constants of about 7 : 1. We discuss the origins of this unusual behaviour in terms of steric interactions within the receptors and their corresponding complexes with guanosine derivatives.

Item Type:

Journal Article

Subjects:

Q Science > QD Chemistry

Divisions:

Faculty of Science > Chemistry

Library of Congress Subject Headings (LCSH):

Binding sites (Biochemistry)

Journal or Publication Title:

Organic & Biomolecular Chemistry

Publisher:

Royal Society of Chemistry

ISSN:

1477-0520

Official Date:

2009

Dates:

Date	Event
2009	Published

Volume:

Vol.7

Number:

No.10

Number of Pages:

Page Range:

pp. 2093-2103

Identifier:

10.1039/b812969j

Status:

Peer Reviewed

Publication Status:

Published

Access rights to Published version:

Restricted or Subscription Access

Description:

Attached MS Excel spreadsheets that enable curve fitting of NMR titration data and calculation of probabilities of binding for molecules above. Monoalkyne is receptor 1, dialkyne is receptor 2, guanosine is guest 3. Also attached are PDF files of Supporting Information here including methods used for NMR and ITC titrations and analysis.

Funder:

University of Warwick. Dept. of Chemistry

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