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Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of individual donors

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Puszyk, William Matthew, Crea, Francesco and Old, R. W. (2009) Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of individual donors. Clinical Biochemistry, Vol.42 (No.7-8). pp. 736-738. doi:10.1016/j.clinbiochem.2008.11.006 ISSN 0009-9120.

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Official URL: http://dx.doi.org/10.1016/j.clinbiochem.2008.11.00...

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Abstract

Objectives: To assess whether different genomic cell-free DNAs are equally abundant in the plasma of individual donors, and any relationship between DNA methylation and representation in plasma.

Design and methods: The concentrations of DNA in plasma were determined by real-time PCR.

Results: Different DNA sequences were not equally represented. The relative abundances were similar in different donors.

Conclusions: Different DNA sequences are not equally abundant in plasma, with no relationship between DNA methylation and abundance. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Item Type: Journal Article
Subjects: R Medicine
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title: Clinical Biochemistry
Publisher: Elsevier Inc.
ISSN: 0009-9120
Official Date: May 2009
Dates:
DateEvent
May 2009UNSPECIFIED
Volume: Vol.42
Number: No.7-8
Number of Pages: 3
Page Range: pp. 736-738
DOI: 10.1016/j.clinbiochem.2008.11.006
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: European Commission for the Special Noninvasive Advances in Fetal and Neonatal Evaluation (SAFE) Network of Excellence
Grant number: LSHB-CT-2004-503243

Data sourced from Thomson Reuters' Web of Knowledge

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