Mayerhofer, Peter U., Cook, Jonathan P., Wahlman, Judit, Pinheiro, Teresa J. T., Moore, Katherine A. H., Lord, Mike (J. Mike), Johnson, Arthur E. and Roberts, Lynne M.. (2009) Ricin A chain insertion into endoplasmic reticulum membranes is triggered by a temperature increase to 37 degrees C. Journal of Biological Chemistry, Vol.284 (No.15). pp. 10232-10242. ISSN 0021-9258

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Abstract

After endocytic uptake by mammalian cells, the heterodimeric plant toxin ricin is transported to the endoplasmic reticulum (ER), where the ricin A chain (RTA) must cross the ER membrane to reach its ribosomal substrates. Here, using gel filtration chromatography, sedimentation, fluorescence, fluorescence resonance energy transfer, and circular dichroism, we show that both fluorescently labeled and unlabeled RTA bind both to ER microsomal membranes and to negatively charged liposomes. The binding of RTA to the membrane at 0-30 degrees C exposes certain RTA residues to the nonpolar lipid core of the bilayer with little change in the secondary structure of the protein. However, major structural rearrangements in RTA occur when the temperature is increased. At 37 degrees C, membrane-bound toxin loses some of its helical content, and its C terminus moves closer to the membrane surface where it inserts into the bilayer. RTA is then stably bound to the membrane because it is nonextractable with carbonate. The sharp temperature dependence of the structural changes does not coincide with a lipid phase change because little change in fluorescence-detected membrane mobility occurred between 30 and 37 degrees C. Instead, the structural rearrangements may precede or initiate toxin retrotranslocation through the ER membrane to the cytosol. The sharp temperature dependence of these changes in RTA further suggests that they occur optimally in mammalian targets of the plant toxin.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Journal of Biological Chemistry
Publisher:	American Society for Biochemistry and Molecular Biology
ISSN:	0021-9258
Official Date:	10 April 2009
Volume:	Vol.284
Number:	No.15
Number of Pages:	11
Page Range:	pp. 10232-10242
Identification Number:	10.1074/jbc.M808387200
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	National Institutes of Health, Wellcome Trust Program, Robert A. Welch Foundation Chair
Grant number:	GM26494, 080566Z/06/Z, BE-0017
URI:	http://wrap.warwick.ac.uk/id/eprint/28209

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