Williams, Jonathan P., Jackson, Hannah and Green, Brian N.. (2009) Hb Belleville [10(A7)AlaThr] affects the determination of Hb A1c by routine cation exchange high performance liquid chromatography. Hemoglobin, Vol.33 (No.1). pp. 45-50. ISSN 0363-0269

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Abstract

When analyzed by routine cation exchange high performance liquid chromatography (HPLC), the Hb A1c peak of a Caucasian diabetic patient from Yorkshire, UK, appeared to be an incompletely resolved doublet. One component (5.5%) eluted at the normal time for Hb A1c, whereas the other component (6.6%) eluted slightly later. The HPLC trace was otherwise normal. Analysis of the diabetic patient's blood and a tryptic digest thereof by electrospray ionization mass spectrometry (ESI-MS) identified the Hb Belleville trait. To relate Hb A1c determined by HPLC to and chain glycation determined by ESI-MS, several normal blood samples (5-14% Hb A1c) were analyzed by both techniques. The Hb A1c levels derived from the and chain glycation levels of the diabetic patient (12.9 and 12.4%, respectively) agreed with the sum of the two peaks (12.1%) in the HPLC trace. Similarly, Hb Belleville was detected and identified in another Caucasian, also from Yorkshire, with normal Hb A1c.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry R Medicine > RB Pathology
Divisions:	Faculty of Science > Chemistry
Journal or Publication Title:	Hemoglobin
Publisher:	Informa Healthcare
ISSN:	0363-0269
Date:	2009
Volume:	Vol.33
Number:	No.1
Number of Pages:	6
Page Range:	pp. 45-50
Identification Number:	10.1080/03630260802625915
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/28538

Data sourced from Thomson Reuters' Web of Knowledge

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