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Methylation of the imprinted GNAS1 gene in cell-free plasma DNA: equal steady-state quantities of methylated and unmethylated DNA in plasma
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Puszyk, William Matthew, Chatha, Kamaljit , Elsenheimer, Selina, Crea, Francesco and Old, Robert W. . (2009) Methylation of the imprinted GNAS1 gene in cell-free plasma DNA: equal steady-state quantities of methylated and unmethylated DNA in plasma. Clinica Chimica Acta, Vol.400 (No.1-2). pp. 107-110. ISSN 0009-8981
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Official URL: http://dx.doi.org/10.1016/j.cca.2008.10.018
Abstract
Background: Genomic DNA sequences in cell-free plasma are biomarkers of cancer prognosis, where characteristic changes in methylation of turnout suppressor or oncogene DNA regions are indicative of changes in gene activity. Also, cell-free fetal DNA can be distinguished, by its methylation status, from the maternal DNA in the plasma of pregnant women, hence providing DNA biomarkers for the proposed minimally-invasive diagnosis of fetal aneuploidies, including Down's syndrome. However, the production and clearance of cell-free DNA from plasma in relation to its methylation status, are poorly understood processes. Methods: We studied the methylation status of DNA derived from the imprinted GNAS1 locus, in cell-free plasma DNA of healthy adults. Heterozygotes were identified that carried the SNP rs1800905 in the imprinted region. The parent-of-origin-dependent DNA methylation was analysed by bisulfite conversion, followed by cloning and sequencing. Results: Genomic DNA molecules derived from both the methylated, maternal, allele and the unmethylated, paternal, allele were found in plasma. Methylated and unmethylated DNA molecules were present in equal numbers. Conclusions: Our data indicate that the methylation status of a DNA sequence has no effect on its steady state concentration in the cell-free DNA component of plasma, in healthy adults. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine |
| Journal or Publication Title: | Clinica Chimica Acta |
| Publisher: | Elsevier BV |
| ISSN: | 0009-8981 |
| Date: | February 2009 |
| Volume: | Vol.400 |
| Number: | No.1-2 |
| Number of Pages: | 4 |
| Page Range: | pp. 107-110 |
| Identification Number: | 10.1016/j.cca.2008.10.018 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Description: | Available online before print; 30 October 2008 |
| Funder: | European Commission for the Special Noninvasive Advances in Fetal and Neonatal Evaluation (SAFE) |
| Grant number: | LSHB-CT-2004-503243 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/28614 |
Data sourced from Thomson Reuters' Web of Knowledge
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