Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Proteomic and metabolomic analysis of cardioprotection: Interplay between protein kinase C epsilon and delta in regulating glucose metabolism of murine hearts

Tools
- Tools
+ Tools

Mayr, Manuel, Liem, David, Zhang, Jun, Li, Xiaohai, Avliyakuiov, Nuraly K., Yang, Jeong In, Young, Glen, Vondriska, Tom M., Ladroue, Christophe, Madhu, Basetti, Griffiths, John R., Gomes, Aldrin, Xu, Qingbo and Ping, Peipei (2009) Proteomic and metabolomic analysis of cardioprotection: Interplay between protein kinase C epsilon and delta in regulating glucose metabolism of murine hearts. Journal of Molecular and Cellular Cardiology, Vol.46 (No.2). pp. 268-277. doi:10.1016/j.yjmcc.2008.10.008 ISSN 0022-2828.

Research output not available from this repository.

Request-a-Copy directly from author or use local Library Get it For Me service.

Official URL: http://dx.doi.org/10.1016/j.yjmcc.2008.10.008

Request Changes to record.

Abstract

We applied a combined proteomic and metabolomic approach to obtain novel mechanistic insights in PKC epsilon-mediated cardioprotection. Mitochondrial and cytosolic proteins from control and transgenic hearts with constitutively active or dominant negative PKC epsilon were analyzed using difference in-gel electrophoresis (DICE). Among the differentially expressed proteins were creatine kinase, pyruvate kinase, lactate clehydrogenase, and the cytosolic isoforms of aspartate amino transferase and malate dehydrogenase, the two enzymatic components of the malate aspartate shuttle, which are required for the import of reducing equivalents from glycolysis across the inner mitochondrial membrane. These enzymatic changes appeared to be dependent on PKC epsilon activity, as they were not observed in mice expressing inactive PKC epsilon. High-resolution proton nuclear magnetic resonance (H-1-NMR) spectroscopy confirmed a pronounced effect of PKC epsilon activity on cardiac glucose and energy metabolism: normoxic hearts with constitutively active PKC epsilon had significantly lower concentrations of glucose, lactate, glutamine and creatine, but higher levels of choline, glutamate and total adenosine nucleotides. Moreover, the depletion of cardiac energy metabolites was slower during ischemia/reperfusion injury and glucose metabolism recovered faster upon reperfusion in transgenic hearts with active PKC epsilon. Notably, inhibition of PKC epsilon resulted in compensatory phosphorylation and mitochondrial translocation of PKC delta. Taken together, our findings are the first evidence that PKC epsilon activity modulates cardiac glucose metabolism and provide a possible explanation for the synergistic effect of PKC delta and PKC epsilon in cardioprotection. (C) 2008 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QM Human anatomy
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science, Engineering and Medicine > Science > Mathematics
Journal or Publication Title: Journal of Molecular and Cellular Cardiology
Publisher: Elsevier Science Ltd. / Academic Press Ltd.
ISSN: 0022-2828
Official Date: February 2009
Dates:
DateEvent
February 2009Published
Volume: Vol.46
Number: No.2
Number of Pages: 10
Page Range: pp. 268-277
DOI: 10.1016/j.yjmcc.2008.10.008
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: British Heart Foundation, Oak Foundation, NIH
Grant number: HL-76526, HL63901, HL654311, HL 80691, HL-80111

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us