O'Rourke, S. (Sean), Wietzorrek, Andreas, Fowler, Kay, Corre, Christophe, Challis, Gregory L. and Chater, K. F.. (2009) Extracellular signalling, translational control, two repressors and an activator all contribute to the regulation of methylenomycin production in Streptomyces coelicolor. Molecular Microbiology, Vol.71 (No.3). pp. 763-778. ISSN 0950-382X

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Abstract

Bioinformatic analysis of the plasmid-linked gene cluster associated with biosynthesis of methylenomycin (Mm) suggested that part of the cluster directs synthesis of a gamma-butyrolactone-like autoregulator. Autoregulator activity could be extracted from culture fluids, but differed from gamma-butyrolactones in being alkali resistant. The activity has recently been shown to comprise a series of novel autoregulator molecules, the methylenomycin furans (termed MMF). MMF autoregulator activity is shown to account for the ability of certain Mm non-producing mutants to act as `secretors' in cosynthesis with other `convertor' mutants. Three genes implicated in MMF biosynthesis are flanked by two regulatory genes, which are related to genes for gamma-butyrolactone-binding proteins. Genetic evidence suggests that these two genes encode components of a hetero-oligomeric repressor of MMF and Mm biosynthesis. The Mm biosynthetic genes themselves depend on the activator gene mmyB, which appears to be repressed by the putative MmyR/MmfR complex until enough MMF accumulates to release repression. The presence of TTA codons in mmyB and the main MMF biosynthetic gene causes Mm production to be dependent on the pleiotropically acting bldA gene, which encodes the tRNA for the rarely used UUA codon.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Streptomyces coelicolor, Biosynthesis, Extracellular space, Cellular signal transduction, Antibiotic-producing organisms, Antibiotics -- Synthesis
Journal or Publication Title:	Molecular Microbiology
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	0950-382X
Date:	February 2009
Volume:	Vol.71
Number:	No.3
Number of Pages:	16
Page Range:	pp. 763-778
Identification Number:	10.1111/j.1365-2958.2008.06560.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	John Innes Foundation
URI:	http://wrap.warwick.ac.uk/id/eprint/28675

Data sourced from Thomson Reuters' Web of Knowledge

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