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Stretching chimeric DNA : a test for the putative S-form
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Whitelam, Stephen, Pronk, Sander and Geissler, Phillip L.. (2008) Stretching chimeric DNA : a test for the putative S-form. The Journal of Chemical Physics, Vol.129 (No.20). ISSN 0021-9606
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Official URL: http://dx.doi.org/10.1063/1.3009266
Abstract
Double-stranded DNA "overstretches" at a pulling force of about 65 pN, increasing in length by a factor of 1.7. The nature of the overstretched state is unknown, despite its considerable importance for DNA's biological function and technological application. Overstretching is thought by some to be a force-induced denaturation and by others to consist of a transition to an elongated, hybridized state called S-DNA. Within a statistical mechanical model, we consider the effect upon overstretching of extreme sequence heterogeneity. "Chimeric" sequences possessing halves of markedly different AT composition elongate under fixed external conditions via distinct, spatially segregated transitions. The corresponding force-extension data vary with pulling rate in a manner that depends qualitatively and strikingly upon whether the hybridized S-form is accessible. This observation implies a test for S-DNA that could be performed in experiment.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QC Physics Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
| Divisions: | Faculty of Science > Chemistry |
| Library of Congress Subject Headings (LCSH): | DNA, Molecular biology, Statistical mechanics |
| Journal or Publication Title: | The Journal of Chemical Physics |
| Publisher: | American Institute of Physics |
| ISSN: | 0021-9606 |
| Date: | 28 November 2008 |
| Volume: | Vol.129 |
| Number: | No.20 |
| Number of Pages: | 8 |
| Identification Number: | 10.1063/1.3009266 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Sixth Framework Programme (European Commission) (FP6), California Institute for Quantitative Biosciences (QB3), National Science Foundation (U.S.) (NSF), Lawrence Berkeley National Laboratory (LBNL), Science Research Investment Fund (SRIF) |
| Grant number: | LSHB-CT2004-005137, DE-AC0205CH11231 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/28680 |
Data sourced from Thomson Reuters' Web of Knowledge
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