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Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin
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Tan, Bee K., Adya, Raghu, Shan, Xiaoye, Syed, Farhatullah, Levandowski, Krzysztof C., O'Hare, J. Paul and Randeva, Harpal S. (2009) Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin. Diabetes Care, Vol.32 (No.1). pp. 129-131. doi:10.2337/dc08-1236
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WRAP_Tan_Ex_vivo_129.full.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (267Kb) | Preview |
Official URL: http://dx.doi.org/10.2337/dc08-1236
Abstract
OBJECTIVE - Lipocalin-2, a novel adipokine, has been shown to be elevated in obese, insulin-resistant, and diabetic Subjects. We therefore sought to study the ex vivo and in vivo effects of insulin on lipocalin-2 levels in humans.
RESEARCH DESIGN AND METHODS - We investigated the in vivo effects of insulin (hyperinsulinemia) on circulating lipocalin-2 levels by enzyme-linked immunosorbent assay via a prolonged insulin-glucose infusion. The ex vivo effect of insulin on adipose tissue lipocalin-2 protein production and secretion into conditioned media was assessed by Western blotting and enzyme-linked immunosorbent assay, respectively.
RESULTS - Hyperinsulinemic induction in human subjects significantly increased circulating lipocalin-2 levels (P < 0.01). Also, in omental adipose tissue explants, insulin caused a significant dose-dependent increase in lipocalin-2 protein production and secretion into conditioned media (P < 0.05, P < 0.01, respectively); these effects were negated by both phosphatidylinositol 3-kinase and mitogen-activated protein kinase kinase inhibitions.
CONCLUSIONS - Lipocalin-2 is upregulated by insulin via phosphatidylinositol 3-kinasc and mitogen-activated protein kinase signaling pathways.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Adipose tissues, Obesity -- Pathophysiology, Insulin resistance | ||||
| Journal or Publication Title: | Diabetes Care | ||||
| Publisher: | American Diabetes Association | ||||
| ISSN: | 0149-5992 | ||||
| Official Date: | January 2009 | ||||
| Dates: |
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| Volume: | Vol.32 | ||||
| Number: | No.1 | ||||
| Number of Pages: | 3 | ||||
| Page Range: | pp. 129-131 | ||||
| DOI: | 10.2337/dc08-1236 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | General Charities of the City of Coventry (GCCC) |
Data sourced from Thomson Reuters' Web of Knowledge
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