The Library
Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin
Tools
Tools
Tools
Tan, Bee K., Adya, Raghu, Shan, Xiaoye, Syed, Farhatullah, Levandowski, Krzysztof C., O'Hare, J. Paul and Randeva, Harpal S.. (2009) Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin. Diabetes Care, Vol.32 (No.1). pp. 129-131. ISSN 0149-5992
|
Text
WRAP_Tan_Ex_vivo_129.full.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (267Kb) | Preview |
Official URL: http://dx.doi.org/10.2337/dc08-1236
Abstract
OBJECTIVE - Lipocalin-2, a novel adipokine, has been shown to be elevated in obese, insulin-resistant, and diabetic Subjects. We therefore sought to study the ex vivo and in vivo effects of insulin on lipocalin-2 levels in humans. RESEARCH DESIGN AND METHODS - We investigated the in vivo effects of insulin (hyperinsulinemia) on circulating lipocalin-2 levels by enzyme-linked immunosorbent assay via a prolonged insulin-glucose infusion. The ex vivo effect of insulin on adipose tissue lipocalin-2 protein production and secretion into conditioned media was assessed by Western blotting and enzyme-linked immunosorbent assay, respectively. RESULTS - Hyperinsulinemic induction in human subjects significantly increased circulating lipocalin-2 levels (P < 0.01). Also, in omental adipose tissue explants, insulin caused a significant dose-dependent increase in lipocalin-2 protein production and secretion into conditioned media (P < 0.05, P < 0.01, respectively); these effects were negated by both phosphatidylinositol 3-kinase and mitogen-activated protein kinase kinase inhibitions. CONCLUSIONS - Lipocalin-2 is upregulated by insulin via phosphatidylinositol 3-kinasc and mitogen-activated protein kinase signaling pathways.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
| Divisions: | Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Adipose tissues, Obesity -- Pathophysiology, Insulin resistance |
| Journal or Publication Title: | Diabetes Care |
| Publisher: | American Diabetes Association |
| ISSN: | 0149-5992 |
| Date: | January 2009 |
| Volume: | Vol.32 |
| Number: | No.1 |
| Number of Pages: | 3 |
| Page Range: | pp. 129-131 |
| Identification Number: | 10.2337/dc08-1236 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | General Charities of the City of Coventry (GCCC) |
| References: | 1. Eckel RH, Grundy SM, Zimmet PZ: The metabolic syndrome. Lancet 365:1415– 1428, 2005 2. Kershaw EE, Flier JS: Adipose tissue as an endocrine organ. J Clin Endocrinol Metab 89:2548–2556, 2004 3. Yan QW, Yang Q, Mody N, Graham TE, Hsu CH, Xu Z, Houstis NE, Kahn BB, Rosen ED: The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance. Diabetes 56:2533–2540, 2007 4. Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, Chow WS, Wat NM, Xu JY, Hoo RL, Xu A: Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans. Clin Chem 53: 34–41, 2007 5. Lewandowski K, Randeva HS, O’Callaghan CJ, Horn R, Medley GF, Hillhouse EW, Brabant G, O’Hare P: Effects of insulin and glucocorticoids on the leptin system are mediated through free leptin. Clin Endocrinol (Oxf) 54:533–539, 2001 6. Tan BK, Chen J, Lehnert H, Kennedy R, Randeva HS: Raised serum, adipocyte and adipose tissue Retinol-Binding Protein 4 (RBP4) in overweight women with polycystic ovary syndrome: effects of gonadal and adrenal steroids. J Clin Endocrinol Metab 92:2764–2772, 2007 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/28769 |
Data sourced from Thomson Reuters' Web of Knowledge
Actions (login required)
 |
View Item |
