Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin
Tan, Bee K., Adya, Raghu, Shan, Xiaoye, Syed, Farhatullah, Levandowski, Krzysztof C., O'Hare, J. Paul and Randeva, Harpal S.. (2009) Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin. Diabetes Care, Vol.32 (No.1). pp. 129-131. ISSN 0149-5992
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Official URL: http://dx.doi.org/10.2337/dc08-1236
OBJECTIVE - Lipocalin-2, a novel adipokine, has been shown to be elevated in obese, insulin-resistant, and diabetic Subjects. We therefore sought to study the ex vivo and in vivo effects of insulin on lipocalin-2 levels in humans.
RESEARCH DESIGN AND METHODS - We investigated the in vivo effects of insulin (hyperinsulinemia) on circulating lipocalin-2 levels by enzyme-linked immunosorbent assay via a prolonged insulin-glucose infusion. The ex vivo effect of insulin on adipose tissue lipocalin-2 protein production and secretion into conditioned media was assessed by Western blotting and enzyme-linked immunosorbent assay, respectively.
RESULTS - Hyperinsulinemic induction in human subjects significantly increased circulating lipocalin-2 levels (P < 0.01). Also, in omental adipose tissue explants, insulin caused a significant dose-dependent increase in lipocalin-2 protein production and secretion into conditioned media (P < 0.05, P < 0.01, respectively); these effects were negated by both phosphatidylinositol 3-kinase and mitogen-activated protein kinase kinase inhibitions.
CONCLUSIONS - Lipocalin-2 is upregulated by insulin via phosphatidylinositol 3-kinasc and mitogen-activated protein kinase signaling pathways.
|Item Type:||Journal Article|
|Subjects:||Q Science > QP Physiology
R Medicine > RC Internal medicine
|Divisions:||Faculty of Medicine > Warwick Medical School > Translational & Systems Medicine > Metabolic and Vascular Health
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Adipose tissues, Obesity -- Pathophysiology, Insulin resistance|
|Journal or Publication Title:||Diabetes Care|
|Publisher:||American Diabetes Association|
|Official Date:||January 2009|
|Number of Pages:||3|
|Page Range:||pp. 129-131|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||General Charities of the City of Coventry (GCCC)|
1. Eckel RH, Grundy SM, Zimmet PZ: The
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