Clewes, E., Edwards, S. G. and Barbara, Dez J.. (2008) Direct molecular evidence supports long-spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids. Plant Pathology, Vol.57 (No.6). pp. 1047-1057. ISSN 0032-0862

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Abstract

There is no published evidence directly supporting the suggestion that long-spored isolates of Verticillium dahliae are interspecific hybrids. Retention in the nuclear genome of long-spored (amphihaploid) isolates of two sequence types where non-hybrid short-spored (haploid) isolates carry only single sequence types would provide such evidence. PCR amplicons for a partial beta-tubulin gene and a 5S rRNA-associated sequence were cloned and sequenced to provide multiple sequences for individual isolates. For the 5S rRNA sequence, two sequence types were found for long-spored isolates, but only one for the haploid isolates. For the beta-tubulin gene, two or three sequence types were found in long-spored isolates, but only one in most haploid isolates. One haploid isolate gave two sequence types, one like that from the other haploid isolates and the second like the short-sequence type from some long-spored isolates. These results support the long-spored isolates being interspecific hybrids between a 'parental' species similar to V. dahliae (haploid) and an unidentified second species. The presence of a sequence type in some long-spored isolates similar to that in one haploid isolate suggests a third 'parental' species may have been involved, possibly via a short-spored isolate that was itself hybrid.

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Item Type:	Journal Article
Subjects:	Q Science > QK Botany S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Verticillium, Verticillium dahliae, Fungal molecular biology, Cruciferae -- Diseases and pests
Journal or Publication Title:	Plant Pathology
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	0032-0862
Date:	December 2008
Volume:	Vol.57
Number:	No.6
Number of Pages:	11
Page Range:	pp. 1047-1057
Identification Number:	10.1111/j.1365-3059.2008.01897.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
URI:	http://wrap.warwick.ac.uk/id/eprint/28960

Data sourced from Thomson Reuters' Web of Knowledge

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