The Library
Direct molecular evidence supports long-spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids
Tools
Tools
Tools
Clewes, E., Edwards, S. G. and Barbara, Dez J. (2008) Direct molecular evidence supports long-spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids. Plant Pathology, Vol.57 (No.6). pp. 1047-1057. doi:10.1111/j.1365-3059.2008.01897.x
Full text not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1111/j.1365-3059.2008.01897.x
Abstract
There is no published evidence directly supporting the suggestion that long-spored isolates of Verticillium dahliae are interspecific hybrids. Retention in the nuclear genome of long-spored (amphihaploid) isolates of two sequence types where non-hybrid short-spored (haploid) isolates carry only single sequence types would provide such evidence. PCR amplicons for a partial beta-tubulin gene and a 5S rRNA-associated sequence were cloned and sequenced to provide multiple sequences for individual isolates. For the 5S rRNA sequence, two sequence types were found for long-spored isolates, but only one for the haploid isolates. For the beta-tubulin gene, two or three sequence types were found in long-spored isolates, but only one in most haploid isolates. One haploid isolate gave two sequence types, one like that from the other haploid isolates and the second like the short-sequence type from some long-spored isolates. These results support the long-spored isolates being interspecific hybrids between a 'parental' species similar to V. dahliae (haploid) and an unidentified second species. The presence of a sequence type in some long-spored isolates similar to that in one haploid isolate suggests a third 'parental' species may have been involved, possibly via a short-spored isolate that was itself hybrid.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) |
||||
| Library of Congress Subject Headings (LCSH): | Verticillium, Verticillium dahliae, Fungal molecular biology, Cruciferae -- Diseases and pests | ||||
| Journal or Publication Title: | Plant Pathology | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 0032-0862 | ||||
| Official Date: | December 2008 | ||||
| Dates: |
|
||||
| Volume: | Vol.57 | ||||
| Number: | No.6 | ||||
| Number of Pages: | 11 | ||||
| Page Range: | pp. 1047-1057 | ||||
| DOI: | 10.1111/j.1365-3059.2008.01897.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
