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Direct molecular evidence supports long-spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids

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Clewes, E., Edwards, S. G. and Barbara, Dez J. (2008) Direct molecular evidence supports long-spored microsclerotial isolates of Verticillium from crucifers being interspecific hybrids. Plant Pathology, Vol.57 (No.6). pp. 1047-1057. doi:10.1111/j.1365-3059.2008.01897.x

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Official URL: http://dx.doi.org/10.1111/j.1365-3059.2008.01897.x

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Abstract

There is no published evidence directly supporting the suggestion that long-spored isolates of Verticillium dahliae are interspecific hybrids. Retention in the nuclear genome of long-spored (amphihaploid) isolates of two sequence types where non-hybrid short-spored (haploid) isolates carry only single sequence types would provide such evidence. PCR amplicons for a partial beta-tubulin gene and a 5S rRNA-associated sequence were cloned and sequenced to provide multiple sequences for individual isolates. For the 5S rRNA sequence, two sequence types were found for long-spored isolates, but only one for the haploid isolates. For the beta-tubulin gene, two or three sequence types were found in long-spored isolates, but only one in most haploid isolates. One haploid isolate gave two sequence types, one like that from the other haploid isolates and the second like the short-sequence type from some long-spored isolates. These results support the long-spored isolates being interspecific hybrids between a 'parental' species similar to V. dahliae (haploid) and an unidentified second species. The presence of a sequence type in some long-spored isolates similar to that in one haploid isolate suggests a third 'parental' species may have been involved, possibly via a short-spored isolate that was itself hybrid.

Item Type: Journal Article
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Faculty of Science > Life Sciences (2010- )
Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH): Verticillium, Verticillium dahliae, Fungal molecular biology, Cruciferae -- Diseases and pests
Journal or Publication Title: Plant Pathology
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 0032-0862
Official Date: December 2008
Dates:
DateEvent
December 2008Published
Volume: Vol.57
Number: No.6
Number of Pages: 11
Page Range: pp. 1047-1057
DOI: 10.1111/j.1365-3059.2008.01897.x
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)

Data sourced from Thomson Reuters' Web of Knowledge

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