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Isolation and characteristics of 10 microsatellite markers from the endangered coconut crab (Birgus latro)

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Gan, Chai-Hsia, Tee, See-Min, Tang, Pei-Ciao, Yang, Jay Ming-Che, Freire, Francis, McGowan, Andrew, Narriman, Jiddawi, Mohammed, Suleiman Mohammed, Hsieh, Hwey-Lian, Chen, Chang-Po, Sheppard, Charles (Charles R. C.) and Chen, Chaolun Allen. (2008) Isolation and characteristics of 10 microsatellite markers from the endangered coconut crab (Birgus latro). Molecular Ecology Resources, Volume 8 (Number 6). pp. 1448-1450. ISSN 1755-098X

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Official URL: http://dx.doi.org/10.1111/j.1755-0998.2008.02330.x

Abstract

The coconut crab (Birgus latro), an endangered marine-dispersed crustacean, is facing severe and probably accelerating population extinction worldwide, but biological information on its conservation remains deficient. In order to reveal the genetic structure of B. latro, 10 microsatellite loci were developed. A high degree of polymorphism was observed with a mean number of alleles per locus of 16.9. The mean expected heterozygosities were also high, ranging from 0.742 to 0.965. The observed heterozygosities ranged from 0.210 to 0.925. Departures from Hardy-Weinberg equilibrium were observed at five loci after the Bonferroni correction. These hypervariable markers will be utilized to study the genetic diversity and conservation of B. latro throughout its distribution range in the Pacific and Indian Oceans.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QL Zoology
Divisions: Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH): Coconut crab, Coenobitidae, Microsatellites (Genetics)
Journal or Publication Title: Molecular Ecology Resources
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 1755-098X
Date: November 2008
Volume: Volume 8
Number: Number 6
Number of Pages: 3
Page Range: pp. 1448-1450
Identification Number: 10.1111/j.1755-0998.2008.02330.x
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Taiwan. Council of Agriculture, Zhongguo ke xue yuan [Chinese Academy of Sciences] (CAS)
Grant number: 0941615101 (COA)
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URI: http://wrap.warwick.ac.uk/id/eprint/29069

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