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Ion mobility augments the utility of mass spectrometry in the identification of human hemoglobin variants
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Williams, Jonathan P., Giles, K. (Kevin), Green, Brian N., Scrivens, James H. and Bateman, R. H. (Robert H.) (2008) Ion mobility augments the utility of mass spectrometry in the identification of human hemoglobin variants. Rapid Communications in Mass Spectrometry, Vol.22 (No.20). pp. 3179-3186. doi:10.1002/rcm.3718
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Official URL: http://dx.doi.org/10.1002/rcm.3718
Abstract
The global dispersion of hemoglobin variants through population migration has precipitated a need for their identification. A particularly effective mass spectrometry (MS)-based procedure involves analysis of the intact globin chains in diluted blood to detect the variant through mass anomalies, followed by location of the variant amino acid residue by direct analysis of the enzymatically digested globins. Here we demonstrate the use of ion mobility separation in combination with this MS procedure to reduce mass spectral complexity. In one example, the doubly charged tryptic peptide from a low abundance variant (4%) occurred at the same m/z value as a singly and a doubly charged interfering ion. In another example, the singly charged tryptic peptide from an alpha-chain variant (26%) occurred at the same m/z value as a doubly charged interfering ion. Ion mobility was used to separate the variant ions from the interfering ions, thus allowing the variant peptides to be observed and sequenced by tandem mass spectrometry. Copyright (C) 2008 John Wiley & Sons, Ltd.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Chemistry |
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| Library of Congress Subject Headings (LCSH): | Ion mobility spectroscopy, Mass spectrometry, Hemoglobin polymorphisms | ||||
| Journal or Publication Title: | Rapid Communications in Mass Spectrometry | ||||
| Publisher: | John Wiley & Sons Ltd. | ||||
| ISSN: | 0951-4198 | ||||
| Official Date: | October 2008 | ||||
| Dates: |
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| Volume: | Vol.22 | ||||
| Number: | No.20 | ||||
| Number of Pages: | 8 | ||||
| Page Range: | pp. 3179-3186 | ||||
| DOI: | 10.1002/rcm.3718 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Wellcome Trust (London, England) |
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