Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

The dynamic architecture of the metabolic switch in Streptomyces coelicolor

Tools
- Tools
+ Tools

Nieselt, Kay, Battke, Florian, Herbig, Alexander, Bruheim, Per, Wentzel, Alexander, Jakobsen, Øyvind M., Sletta, Håvard, Alam, Mohammad T., Merlo, Maria E., Moore, Jonathan D. et al.
(2010) The dynamic architecture of the metabolic switch in Streptomyces coelicolor. BMC Genomics, Vol.11 (Article 10). doi:10.1186/1471-2164-11-10

[img] PDF
WRAP_Challis_Dynamic_Architecture.pdf - Requires a PDF viewer.

Download (961Kb)
Official URL: http://dx.doi.org/10.1186/1471-2164-11-10

Request Changes to record.

Abstract

Background: During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples.
Results: Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before the classically defined transition phase where the switch from primary to secondary metabolism was expected. The main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are delayed relative to the metabolic rearrangements. Furthermore, global variation in morphogenesis genes indicates an involvement of cell differentiation pathways in the decision phase leading up to the commitment to antibiotic biosynthesis.
Conclusions: Our study provides the first detailed insights into the complex sequence of early regulatory events during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Faculty of Science > Chemistry
Faculty of Science > Centre for Systems Biology
Library of Congress Subject Headings (LCSH): Streptomyces coelicolor, Soil microbiology -- Research, Microbial metabolism -- Regulation, Antibiotics -- Synthesis, Bacteria -- Morphogenesis
Journal or Publication Title: BMC Genomics
Publisher: BioMed Central Ltd.
ISSN: 1471-2164
Official Date: 6 January 2010
Dates:
DateEvent
6 January 2010Published
Volume: Vol.11
Number: Article 10
DOI: 10.1186/1471-2164-11-10
Status: Peer Reviewed
Access rights to Published version: Open Access
Funder: European Commission (EC), Norges forskningsråd [Norwegian Research Council]
Grant number: GEN2006- 27745-E/SYS: P-UK-01-11-3i (SySMO), 181840/I30 (NF)

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us