Sauter, Alexander, Bas-Orth, Daniel, Stern-Straeter, Jens, Barnes, J. (James), Soulsby, Hannah, Hoermann, Karl and Naim, Ramin. (2008) Effect of vascular cell adhesion molecule 1 on fibroblasts in human eosinophilic chronic rhinosinusitis. International Journal of Molecular Medicine, Vol.22 (No.4). pp. 441-446. ISSN 1107-3756

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Abstract

The pathogenesis of eosinophilic chronic rhinosinusitis (ECRS) is still unclear. Paranasal mucosa inflammation is thought to be related to eosinophilic infiltration. This infiltration seems to induce changes in the expression of cell adhesion molecules such as vascular cell adhesion molecule 1 (VCAM-1). The E-eadherin-beta-catenin complex maintains the integrity of the epithelium. Downregulation of beta-catenin and E-cadherin is a pivotal factor for progressive cell growth. This study aimed to assess which cytokines regulate the expression of the adhesion molecule E-cadherin and the multi-functional protein B-catenin, which plays a key role in cadherin-mediated anchoring in ECRS. Cultured ECRS specimens were incubated with human VCAM-1. After a period of up to 72 h, expression of E-cadherin and B-catenin was determined using cytokine immunoassay and immunohistochemistry. In ECRS, significant increases in E-cadherin expression were found in fibroblast cell cultures. Stimulation with VCAM-1 did not produce a significant alteration in the expression of the adherens junction protein B-catenin. In addition, VCAM-1 did not decrease the levels of membrane staining for adherens junction proteins. The selective increase in E-cadherin expression in eosinophilic fibroblast cultures might be explained by a higher concentration of the Th2-type cytokines in these cultures. The tissue remodelling observed during chronic eosinophilic inflammation offers new insight into the pathogenesis of ECRS.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology R Medicine > RF Otorhinolaryngology
Divisions:	Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Cell adhesion molecules, Eosinophils, Sinusitis, Paranasal sinuses -- Endoscopic surgery, Cadherins
Journal or Publication Title:	International Journal of Molecular Medicine
Publisher:	Spandidos Publications
ISSN:	1107-3756
Date:	October 2008
Volume:	Vol.22
Number:	No.4
Number of Pages:	6
Page Range:	pp. 441-446
Identification Number:	10.3892/ijmm_00000040
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
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URI:	http://wrap.warwick.ac.uk/id/eprint/29223

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