Grime, John M. A., Edwards, Martin A., Rudd, Nicola C. and Unwin, Patrick R.. (2008) Quantitative visualization of passive transport across bilayer lipid membranes. Proceedings of the National Academy of Sciences of the United States of America, Vol.105 (No.38). pp. 14277-14282. ISSN 0027-8424

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Abstract

The ability to predict and interpret membrane permeation coefficients is of critical importance, particularly because passive transport is crucial for the effective delivery of many pharmaceutical agents to intracellular targets. We present a method for the quantitative measurement of the permeation coefficients of protonophores by using laser confocal scanning microscopy coupled to microelectrochemistry, which is amenable to precise modeling with the finite element method. The technique delivers well defined and high mass transport rates and allows rapid visualization of the entire pH distribution on both the cis and trans side of model bilayer lipid membranes (BLMs). A homologous series of carboxylic acids was investigated as probe molecules for BLMs composed of soybean phosphatidylcholine. Significantly, the permeation coefficient decreased with acyl tail length contrary to previous work and to Overton's rule. The reasons for this difference are considered, and we suggest that the applicability of Overton's rule requires re-evaluation.

Item Type:	Journal Article
Subjects:	Q Science > QC Physics Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Ultramicroelectrodes, Confocal microscopy, Finite element method, Bilayer lipid membranes, Biological transport
Journal or Publication Title:	Proceedings of the National Academy of Sciences of the United States of America
Publisher:	National Academy of Sciences
ISSN:	0027-8424
Date:	23 September 2008
Volume:	Vol.105
Number:	No.38
Number of Pages:	6
Page Range:	pp. 14277-14282
Identification Number:	10.1073/pnas.0803720105
Status:	Not Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Engineering and Physical Sciences Research Council (EPSRC), University of Warwick
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URI:	http://wrap.warwick.ac.uk/id/eprint/29289

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