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A single amino acid change in a geminiviral Rep protein differentiates between triggering a plant defence response and initiating viral DNA replication
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Jin, Mingfei, Li, Chunyang, Shi, Yan, Ryabov, Eugene V., Huang, Jing, Wu, Zirong, Fan, Zaifeng and Hong, Yiguo (2008) A single amino acid change in a geminiviral Rep protein differentiates between triggering a plant defence response and initiating viral DNA replication. Journal of General Virology, Vol.89 (Part 10). pp. 2636-2641. doi:10.1099/vir.0.2008/001966-0 ISSN 0022-1317.
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Official URL: http://dx.doi.org/10.1099/vir.0.2008/001966-0
Abstract
We have devised an in planta system for functional analysis of the replication-associated protein (Rep) of African cassava mosaic virus (ACMV). Using this assay and PCR-based random mutagenesis, we have identified an ACMV Rep mutant that failed to trigger the hypersensitive response (HR), but had an enhanced ability to initiate DNA replication. The mutant Rep-green fluorescent protein (GFP) fusion protein was localized to the nucleus. Sequence analysis showed that the mutated Rep gene had three nucleotide changes (A6-->T, T375-->G and G852-->A); only the A6-->T transversion resulted in an amino acid substitution (Arg to Ser), which is at the second residue in the 358 amino acid ACMV Rep protein. Our results indicate that a single amino acid can alter the differential ability of ACMV Rep to trigger the host-mediated HR defence mechanism and to initiate viral DNA replication. The implications of this finding are discussed in the context of plant-virus interactions.
Item Type: | Journal Article | ||||
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Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Journal of General Virology | ||||
Publisher: | Society for General Microbiology | ||||
ISSN: | 0022-1317 | ||||
Official Date: | October 2008 | ||||
Dates: |
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Volume: | Vol.89 | ||||
Number: | Part 10 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 2636-2641 | ||||
DOI: | 10.1099/vir.0.2008/001966-0 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Royal Society (Great Britain) | ||||
Grant number: | 2007/R1/Hong, 2006R2/China/KCW/Hong |
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