A single amino acid change in a geminiviral Rep protein differentiates between triggering a plant defence response and initiating viral DNA replication
Jin, Mingfei, Li, Chunyang, Shi, Yan, Ryabov, Eugene , Huang, Jing, Wu, Zirong, Fan, Zaifeng and Hong, Yiguo. (2008) A single amino acid change in a geminiviral Rep protein differentiates between triggering a plant defence response and initiating viral DNA replication. Journal of General Virology, Vol.89 (Part 10). pp. 2636-2641. ISSN 0022-1317Full text not available from this repository.
Official URL: http://dx.doi.org/10.1099/vir.0.2008/001966-0
We have devised an in planta system for functional analysis of the replication-associated protein (Rep) of African cassava mosaic virus (ACMV). Using this assay and PCR-based random mutagenesis, we have identified an ACMV Rep mutant that failed to trigger the hypersensitive response (HR), but had an enhanced ability to initiate DNA replication. The mutant Rep-green fluorescent protein (GFP) fusion protein was localized to the nucleus. Sequence analysis showed that the mutated Rep gene had three nucleotide changes (A6-->T, T375-->G and G852-->A); only the A6-->T transversion resulted in an amino acid substitution (Arg to Ser), which is at the second residue in the 358 amino acid ACMV Rep protein. Our results indicate that a single amino acid can alter the differential ability of ACMV Rep to trigger the host-mediated HR defence mechanism and to initiate viral DNA replication. The implications of this finding are discussed in the context of plant-virus interactions.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
|Divisions:||Faculty of Science > Life Sciences (2010- )|
|Journal or Publication Title:||Journal of General Virology|
|Publisher:||Society for General Microbiology|
|Number of Pages:||6|
|Page Range:||pp. 2636-2641|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Royal Society (Great Britain)|
|Grant number:||2007/R1/Hong, 2006R2/China/KCW/Hong|
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