Damianoglou, Angeliki, Crust, Edward J., Hicks, Matthew R., Howson, Suzanne E., Knight, Alex, Ravi, Jascindra, Scott, Peter, 1965 Dec. 10- and Rodger, Alison. (2008) A new reference material for UV-visible circular dichroism spectroscopy. Chirality, Vol.20 (No.9, Sp.Iss.SI). pp. 1029-1038. ISSN 0899-0042

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Abstract

To obtain accurate and consistent measurements from circular dichroism (CD) instruments over time and from different laboratories, it is important that they are properly calibrated. The characteristics of the available reference materials are not ideal to ensure proper calibration as they typically only give peaks in one or two spectral regions, and often have issues concerning purity and stability. Currently either camphor sulfonic acid or ammonium camphor sulfonate are used. The latter can be an unstable, slightly hygroscopic secondary standard compound with only one characterized CD band. The former is the very hygroscopic primary standard for which only one enantiomer is readily available. We have synthesized a new reference material for CD, Na[Co(EDDS)]center dot H2O (EDDS = N,N-ethylenediaminedisuccinic acid) which addresses these problems. It is extremely stable and available in both enantiomeric forms. The CD spectrum of Na[Co(EDDS)]center dot H2O has nine distinct peaks between 180 and 599 nm. It thus fulfils the principal requirements for CD calibration chemical standards and has the potential to be used to ensure good practice in the measurement of CD data, providing two spectra of equal magnitude and opposite sign for a given concentration and path length. We have carried out an interlaboratory comparison using this material and show how it can be used to improve CD comparability between laboratories. A fitting algorithm has been developed to assess CD spectropolarimeter performance between 750 and 178 nm. This could be the basis of a formal quality control process once criteria for performance have been decided.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Circular dichroism, Calibration, Enantiomers, Chirality
Journal or Publication Title:	Chirality
Publisher:	John Wiley & Sons, Inc.
ISSN:	0899-0042
Date:	October 2008
Volume:	Vol.20
Number:	No.9, Sp.Iss.SI
Number of Pages:	10
Page Range:	pp. 1029-1038
Identification Number:	10.1002/chir.20566
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Great Britain. National Measurement System Directorate, Engineering and Physical Sciences Research Council (EPSRC)
Version or Related Resource:	Presented at: 11th International Conference on Circular Dichroism, Groningen, Netherlands, 2-6 Sep 2007
Conference Paper Type:	Paper
Type of Event:	Conference
URI:	http://wrap.warwick.ac.uk/id/eprint/29309

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