Hilton, Sally, Kemp, E. (Elizabeth), Keane, G. (Gary) and Winstanley, Doreen. (2008) A bacmid approach to the genetic manipulation of granuloviruses. Journal of Virological Methods, Vol.152 (No.1-2). pp. 56-62. ISSN 0166-0934

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Abstract

A Cydia pomonella granulovirus (CpGV) bacmid has been constructed, which allows rapid and efficient production of recombinant baculoviruses in Escherichia coli. An 8.6 kbp bacterial DNA cassette derived from the AcMNPV Bac-to-Bac (R) system was ligated into a unique Pacl restriction site within an intergenic region flanking the DNA ligase gene of the CpGV genome. The CpGV bacmids produced in E. coli were transfected into a CpGV-permissive C. pomonella cell line and the transfected cells fed to larvae to amplify the virus. The enhanced green fluorescent protein (EGFP) gene under the constitutive Drosophila heat-shock promoter was transposed into the mini-attTn7 transposition site, using a modified pFASTBAC (TM) donor plasmid, to generate a recombinant CpGV bacmid which caused infected larvae to glow under UV light. Targeted homologous recombination was also achieved in a recombinant proficient E. coli strain (BJ5183). A chloramphenicol acetyl transferase (CAT) gene replaced the cathepsin (v-cath) gene in the bacmid to produce a v-cath-deletion mutant. This is the first published report of a granulovirus bacmid, which will allow easy manipulation of the CpGV genome, enabling future studies on granulovirus genes and biology. (C) 2008 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Baculoviruses -- Genetics, Codling moth -- Pathogens, Microbial genetic engineering
Journal or Publication Title:	Journal of Virological Methods
Publisher:	Elsevier BV
ISSN:	0166-0934
Date:	September 2008
Volume:	Vol.152
Number:	No.1-2
Number of Pages:	7
Page Range:	pp. 56-62
Identification Number:	10.1016/j.jviromet.2008.05.015
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
References:	Agrawal, N., Malhotra, P., Bhatnagar, R.K., 2004. siRNA-directed silencing of transgene expressed in cultured insect cells. Biochem. Biophys. Res. Commun. 320, 428–434. Bideshi, D.K., Federici, B.A., 2000. The Trichoplusia ni granulovirus helicase is unable to support replication of Autographa californica multicapsid nucleopolyhedrovirus in cells and larvae of T. ni. J. Gen. Virol. 81, 1593–1599. Chavira, R., Burnett, T.J., Hageman, J.H., 1984. Assaying proteinases with Azocoll. Anal. Biochem. 136, 446–450. Croizier, G., Croizier, L., Quiot, J.M., Lereclus, D., 1988. Recombination of Autographa californica and Rachiplusia ou nuclear polyhedrosis viruses in Galleria mellonella L. J. Gen. Virol. 69, 177–185. Crook, N.E., Payne, C.C., 1980. Comparison of three methods of ELISA for baculoviruses. J. Gen. Virol. 46, 26–37. Crook, N.E., Spencer, R.A., Payne, C.C., Leisy, D.J., 1985. Variation in Cydia pomonella granulosis virus isolates and physical maps of the DNA from 3 variants. J. Gen. Virol. 66, 2423–2430. Crook, N.E., James, J.D., Smith, I.R.L., Winstanley, D., 1997. Comprehensive physical map of the Cydia pomonella granulovirus genome and sequence analysis of the granulin gene region. J. Gen. Virol. 78, 965–974. Cross, J.V., Solomon, M.G., Chandler, D., Jarrett, P., Richardson, P.N., Winstanley, D., Bathon, H., Huber, J., Keller, B., Langenbruch, G.A., Zimmerman, G., 1999. Biocontrol of pests of apples and pears in northern and central Europe: 1. Microbial agents and nematodes. Biosci. Biotechnol. Biochem. 9, 125–149. Guennelon, G., Audemard, H., Fremond, J.C., El Idrissi Ammari, M.A., 1981. Progre` s re´ alise´ s dans l’elevage permanent du Carpocapse Laspeyresia pomonella L. sur milieu artificiel. Agronomie 1, 59–64. Hanahan, D., 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557–580. Hawtin, R.E., Zarkowska, T., Arnold, K., Thomas, C.J., Gooday, G.W., King, L.A., Kuzio, J.A., Possee, R.D., 1997. Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virusencoded chitinase and cathepsin genes. Virology 238, 243–253. Hilton, S., Winstanley, D., 2007. Identification and functional analysis of the origins of DNA replication in the Cydia pomonella granulovirus genome. J. Gen. Virol. 88, 1496–1504. Hilton, S., 2008. Baculoviridae: granulovirus, molecular biology, in: Mahy, B.W.J., van Regenmortel, M. (Eds.), Encyclopedia of Virology, 3rd ed., Academic Press. Hou, S., Chen, X., Wang, H., Tao, M., Hu, Z., 2002. Efficient method to generate homologous recombinant baculovirus genomes in E-coli. Biotechniques 32, 783–788. Jarvis, D.L., 1997. Baculovirus expression vectors. In: Miller, L.K. (Ed.), The Baculoviruses. Plenum Publishing Corporation, New York, pp. 389–431. Lin, G.Y., Blissard, G.W., 2002. Analysis of an Autographa californica nucleopolyhedrovirus lef-11 knockout: LEF-11 is essential for viral DNA replication. J. Virol. 76, 2770–2779. Luckow, V.A., Lee, S.C., Barry, G.F., Olins, P.O., 1993. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J. Virol. 67, 4566–4579. Lung, O.,Westenberg, M., Vlak, J.M., Zuidema, D., Blissard, G.W., 2002. Pseudotyping Autographa californica multicapsid nucleopolyhedrovirus, AcMNPV: F proteins from group II NPVs are functionally analogous to AcMNPV GP64. J. Virol. 76, 5729–5736. Luque, T., Finch, R., Crook, N., O’Reilly, D.R., Winstanley, D., 2001. The complete sequence of Cydia pomonella granulovirus genome. J. Gen. Virol. 82, 2531–2547. Means, J.C., Muro, I., Clem, R.J., 2003. Silencing of the baculovirus Op-iap3 gene by RNA interference reveals that it is required for prevention of apoptosis during Orgyia pseudotsugataMNucleopolyhedrovirus Infection of Ld652Y Cells. J. Virol. 77, 4481–4488. Motohashi, T., Shimojima, T., Fukagawa, T., Maenaka, K., Park, E.Y., 2005. Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system. Biochem. Biophys. Res. Commun. 326, 564–569. Ohkawa, T., Majima, K., Maeda, S., 1994. A cysteine proteinase encoded by the Baculovirus Bombyx mori nuclear polyhedrosis virus. J. Virol. 68, 6619–6625. Pijlman, G.P., Dortmans, J.C.F.M., Vermeesch, A.M.G., Yang, K., Martens, D.E., Goldbach, R.W., Vlak, J.M., 2002. Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses. J. Virol. 76, 5605–5611. Skuratovskaya, I.N., Strokovskaya, L.I., Zherebtsova, E.N., Gudz-Gorban, A.P., 1977. Supercoiled DNA of nuclear polyhedrosis virus of Galleria mellonella L. Arch. Virol. 53, 79–86. Smith, I.R.L., Crook, N.E., 1988. In vivo isolation of baculovirus genotypes. Virology 166, 240–244. Theilmann, D.A., Blissard, G.W., Bonning, B., Jehle, J., O’Reilly, D.R., Rohrmann, G.F., Theim, S., Vlak, J.M., 2005. Baculoviridae. In: Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., Ball, L.A. (Eds.), Virus Taxonomy—Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, pp. 177–185. Vanarsdall, A.L., Okano, K., Rohrmann, G.F., 2004. Characterization of a baculovirus with a deletion of vlf-1. Virology 326 (1), 191–201. Wang, H.Z., Deng, F., Pijlman, G.P., Chen, X.W., Sun, X.L., Vlak, J.M., Hu, Z.H., 2003. Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome. Virus Res. 97, 57–63. Winstanley, D., Crook, N.E., 1993. Replication of Cydia pomonella granulosis virus in cell cultures. J. Gen. Virol. 74, 1599–1609. Wormleaton, S. L., 2000. Molecular and biological studies of fast and slow killing granuloviruses. Ph.D. Thesis. University ofWarwick. Wu,W.W.,Wang, J.W., Deng, R.Q.,Wang, X.Z., He, X.L., Long, Q.X., 2003. An efficient method for precise gene substitution in the AcMNPV genome by homologous recombination in E. coli. J. Virol. Methods 113, 95–101. Zhang, Y.M., Buchholz, F., Muyrers, J.P.P., Stewart, A.F., 1998. A new logic for DNA engineering using recombination in Escherichia coli. Nat. Genet. 20, 123–128.
URI:	http://wrap.warwick.ac.uk/id/eprint/29439

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