Krishnan, Nithya S., Fleetwood, Paul, Higgins, Rob (Robert Mcrorie), Hathaway, M. (Mark), Zehnder, Daniel, Mitchell, Daniel Anthony, Hamer, Rizwan, Fletcher, S. (Simon), Lam, For Tai, Kashi, Habib, Tan, Lam Chin, Imray, C. (Chris) and Briggs, D. (David). (2008) Application of flow cytometry to monitor antibody levels in ABO incompatible kidney transplantation. Transplantation, Vol.86 (No.3). pp. 474-477. ISSN 0041-1337

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Abstract

Current methods of measuring ABO antibody levels based on the hemagglutination (HA) titers have the disadvantages of relatively poor reproducibility and do not offer fine discrimination of antibody concentration. We therefore developed a simple and rapid method of measuring ABO antibody levels using flow cytometry (FC). For validation, we analyzed plasma samples from 79 blood donors. Both IgM and IgG were detected and measured with IgG essentially restricted blood group O donors. Forty-two successive samples were collected from a patient with blood group O undergoing antibody removal and subsequent transplantation from a group A2 donor and tested by both HA and FC. Changes in IgG measured by FC (relative median fluorescence) correlated well with HA titers and importantly rejection episodes were preempted by a rising relative median fluorescence. The method allowed quantitative discrimination in the range of antibody levels relevant to ABO incompatible transplantation and has the advantages over HA of objective measurement and reproducibility.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > RD Surgery
Divisions:	Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Flow cytometry, Blood groups -- ABO system, Transplantation immunology, Kidneys -- Transplantation
Journal or Publication Title:	Transplantation
Publisher:	Lippincott Williams & Wilkins
ISSN:	0041-1337
Date:	15 August 2008
Volume:	Vol.86
Number:	No.3
Number of Pages:	4
Page Range:	pp. 474-477
Identification Number:	10.1097/TP.0b013e31817c4c4c
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	University Hospitals Coventry and Warwickshire NHS Trust, NHS Blood and Transplant
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URI:	http://wrap.warwick.ac.uk/id/eprint/29492

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