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Enzyme-independent, orientation-selective conjugation of whole human complement C3 to protein surfaces
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Mitchell, Daniel A., Ilyas, Rebecca, Dodds, Alister W. and Sim, Robert B. (2008) Enzyme-independent, orientation-selective conjugation of whole human complement C3 to protein surfaces. Journal of Immunological Methods, Vol.337 (No.1). pp. 49-54. doi:10.1016/j.jim.2008.05.011
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Official URL: http://dx.doi.org/10.1016/j.jim.2008.05.011
Abstract
Complement C3 is a central component of the humoral immune system. Upon triggering of the complement cascade, proteolytic fragments of C3 mediate important processes such as opsonization and lymphocyte activation. C3 possesses an internal thioester that mediates covalent attachment of proteolytically activated C3 to target surfaces. Treatment of native C3 with methylamine cleaves the thioester bond and exposes a free sulfhydryl group at the target-binding face of the protein. Through the use of sulfhydryl-reactive heterobifunctional Crosslinking and biotinylation reagents, we demonstrate the capacity to form stable, multimeric whole human C3-protein conjugates in a fashion reflecting the orientation of physiologically-activated C3. We speculate that this C3 conjugation strategy presents a route for targeting dendritic cells and macrophages. In addition, manipulation of the thioester bond could enhance the study of biological roles of C3 and related proteins such as C4, and also of transmissible agents that exploit complement function such as prions. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology > QR180 Immunology |
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Complement (Immunology), Immunological adjuvants, Biochemistry | ||||
| Journal or Publication Title: | Journal of Immunological Methods | ||||
| Publisher: | Elsevier BV | ||||
| ISSN: | 0022-1759 | ||||
| Official Date: | 20 August 2008 | ||||
| Dates: |
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| Volume: | Vol.337 | ||||
| Number: | No.1 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 49-54 | ||||
| DOI: | 10.1016/j.jim.2008.05.011 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | European Union (EU), University of Warwick | ||||
| Grant number: | FOOD-CT-2006-023144 (EU) |
Data sourced from Thomson Reuters' Web of Knowledge
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