Takahashi, M. (Maho), Nakai, Madoka, Nakanishi, Kazuko, Sato, Takeru, Hilton, Sally, Winstanley, Doreen and Kunimi, Yasuhisa. (2008) Genetic and biological comparisons of four nucleopolyhedrovirus isolates that are infectious to Adoxophyes honmai (Lepidoptera : Tortricidae). Biological Control, Vol.46 (No.3). pp. 542-546. ISSN 1049-9644

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Abstract

The smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae), is one of the most important pests of tea plants in Japan. Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) isolates from Tsukuba (AdhoNPV-Ts) and Tokyo (AdhoNPV-To), Japan, and Adoxophyes orana nucleopolyhedrovirus (AdorNPV) isolates from England (AdorNPV-En) and the Netherlands (AdorNPV-Ne) were subjected to genetic and biological comparisons to select a candidate NPV isolate to control A. honmai. Restriction endonuclease (REN) analysis demonstrated that AdhoNPV-Ts and AdhoNPV-To had similar REN patterns, whereas AdorNPV-En and AdorNPV-Ne exhibited different REN patterns from each other as well as those of AdhoNPV-Ts and AdhoNPV-To. Bioassays with fourth-instar A. honmai larvae showed that AdorNPV-En was most pathogenic, with the lowest LD50 of 37 occlusion bodies (OBs) per larva. When A. honmai neonates were inoculated with each isolate, most larvae infected with AdhoNPV-Ts and AdhoNPV-To were killed in the final (fifth)-instar, whereas larvae infected with AdorNPV-Ne were killed at every instar and larvae infected with AdorNPV-En were killed at the first- to third-instar. AdorNPV-En or AdhoNPV-Ts fed to neonates had the shortest or longest killing times, respectively, with ST50 values of 6 and 19 days. AdhoNPV-To and AdorNPV-Ne had intermediate killing times. The OB yield per larva of AdhoNPV-Ts and AdhoNPV-To was significantly higher than that of AdorNPV-En and AdorNPV-Ne. Our results suggest that AdorNPV-En is suitable as an inundative agent because it is a quick-killing, highly virulent NPV, and AdhoNPV-Ts and AdhoNPV-To are more appropriately used as inoculative agents because of their high OB production. (C) 2008 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Baculoviruses, Nucleopolyhedroviruses, Tea -- Diseases and pests
Journal or Publication Title:	Biological Control
Publisher:	Elsevier
ISSN:	1049-9644
Official Date:	September 2008
Volume:	Vol.46
Number:	No.3
Number of Pages:	5
Page Range:	pp. 542-546
Identification Number:	10.1016/j.biocontrol.2008.06.003
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Japan. Nōrin Suisanshō [Ministry of Agriculture, Forestry, and Fisheries] (MAFF)
URI:	http://wrap.warwick.ac.uk/id/eprint/29519

Data sourced from Thomson Reuters' Web of Knowledge

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