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Genetic and biological comparisons of four nucleopolyhedrovirus isolates that are infectious to Adoxophyes honmai (Lepidoptera : Tortricidae)
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Takahashi, M. (Maho), Nakai, Madoka, Nakanishi, Kazuko, Sato, Takeru, Hilton, Sally, Winstanley, Doreen and Kunimi, Yasuhisa (2008) Genetic and biological comparisons of four nucleopolyhedrovirus isolates that are infectious to Adoxophyes honmai (Lepidoptera : Tortricidae). Biological Control, Vol.46 (No.3). pp. 542-546. doi:10.1016/j.biocontrol.2008.06.003
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Official URL: http://dx.doi.org/10.1016/j.biocontrol.2008.06.003
Abstract
The smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae), is one of the most important pests of tea plants in Japan. Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) isolates from Tsukuba (AdhoNPV-Ts) and Tokyo (AdhoNPV-To), Japan, and Adoxophyes orana nucleopolyhedrovirus (AdorNPV) isolates from England (AdorNPV-En) and the Netherlands (AdorNPV-Ne) were subjected to genetic and biological comparisons to select a candidate NPV isolate to control A. honmai. Restriction endonuclease (REN) analysis demonstrated that AdhoNPV-Ts and AdhoNPV-To had similar REN patterns, whereas AdorNPV-En and AdorNPV-Ne exhibited different REN patterns from each other as well as those of AdhoNPV-Ts and AdhoNPV-To. Bioassays with fourth-instar A. honmai larvae showed that AdorNPV-En was most pathogenic, with the lowest LD50 of 37 occlusion bodies (OBs) per larva. When A. honmai neonates were inoculated with each isolate, most larvae infected with AdhoNPV-Ts and AdhoNPV-To were killed in the final (fifth)-instar, whereas larvae infected with AdorNPV-Ne were killed at every instar and larvae infected with AdorNPV-En were killed at the first- to third-instar. AdorNPV-En or AdhoNPV-Ts fed to neonates had the shortest or longest killing times, respectively, with ST50 values of 6 and 19 days. AdhoNPV-To and AdorNPV-Ne had intermediate killing times. The OB yield per larva of AdhoNPV-Ts and AdhoNPV-To was significantly higher than that of AdorNPV-En and AdorNPV-Ne. Our results suggest that AdorNPV-En is suitable as an inundative agent because it is a quick-killing, highly virulent NPV, and AdhoNPV-Ts and AdhoNPV-To are more appropriately used as inoculative agents because of their high OB production. (C) 2008 Elsevier Inc. All rights reserved.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
| Library of Congress Subject Headings (LCSH): | Baculoviruses, Nucleopolyhedroviruses, Tea -- Diseases and pests | ||||
| Journal or Publication Title: | Biological Control | ||||
| Publisher: | Elsevier | ||||
| ISSN: | 1049-9644 | ||||
| Official Date: | September 2008 | ||||
| Dates: |
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| Volume: | Vol.46 | ||||
| Number: | No.3 | ||||
| Number of Pages: | 5 | ||||
| Page Range: | pp. 542-546 | ||||
| DOI: | 10.1016/j.biocontrol.2008.06.003 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Japan. Nōrin Suisanshō [Ministry of Agriculture, Forestry, and Fisheries] (MAFF) |
Data sourced from Thomson Reuters' Web of Knowledge
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