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The Tat system proofreads FeS protein substrates and directly initiates the disposal of rejected molecules
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Matos, Cristina F. R. O., Robinson, Colin and Di Cola, Alessandra (2008) The Tat system proofreads FeS protein substrates and directly initiates the disposal of rejected molecules. EMBO Journal, Vol.27 (No.15). pp. 2055-2063. doi:10.1038/emboj.2008.132 ISSN 0261-4189.
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Official URL: http://dx.doi.org/10.1038/emboj.2008.132
Abstract
The twin-arginine translocation (Tat) system transports folded proteins across the bacterial plasma membrane, including FeS proteins that receive their cofactors in the cytoplasm. We have studied two Escherichia coli Tat substrates, NrfC and NapG, to examine how, or whether, the system exports only correctly folded and assembled FeS proteins. With NrfC, substitutions in even one of four predicted FeS centres completely block export, indicating an effective proofreading activity. The FeS mutants are rapidly degraded but only if they interact with the Tat translocon; they are stable in a tat deletion strain and equally stable in wild-type cells if the signal peptide twin-arginine motif is removed to block targeting. Basically similar results are obtained with NapG. The Tat apparatus thus proofreads these substrates and directly initiates the turnover of rejected molecules. Turnover of mutated FeS substrates is completely dependent on the TatA/E subunits that are believed to be involved in the late stages of translocation, and we propose that partial translocation triggers substrate turnover within an integrated quality control system for FeS proteins.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Proteins, Proteins -- Physiological transport, Membranes (Biology), Biological transport | ||||
Journal or Publication Title: | EMBO Journal | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 0261-4189 | ||||
Official Date: | 6 August 2008 | ||||
Dates: |
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Volume: | Vol.27 | ||||
Number: | No.15 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 2055-2063 | ||||
DOI: | 10.1038/emboj.2008.132 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), European Union (EU) | ||||
Grant number: | LSHG-CT-2004-005257 (EU) |
Data sourced from Thomson Reuters' Web of Knowledge
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