Regulation of matrix metalloproteinases (MMP)-2/-9 expression in eosinophilic chronic rhinosinusitis - Cell culture by interleukin-5 and-13?
Sauter, Alexander, Stern-Straeter, Jens, Sodha, Selina, Hoermann, Karl and Naim, Ramin. (2008) Regulation of matrix metalloproteinases (MMP)-2/-9 expression in eosinophilic chronic rhinosinusitis - Cell culture by interleukin-5 and-13? In Vivo, Vol.22 (No.4). pp. 415-421. ISSN 0258-851XFull text not available from this repository.
Official URL: http://iv.iiarjournals.org/content/22/4/415.abstra...
Background: Eosinophils are a prominent immunological feature of chronic rhinosinusitis (CRS). Cytokines in the respiratory mucosa may be the key to upper airway pathophysiology. Matrix metalloproteinases (MMP) represent an entire group of Zn2+ dependent endopeptidases with the potential to alter the extracellular matrix (ECM). In this study epithelial cultures of CRS were treated with interleukin (IL)-5 or IL-13 and subsequent levels of metalloproteinases were determined. Materials and Methods: The cells for CRS culture were obtained from patients undergoing functional endoscopic sinus surgery. After 8-72 hours incubation with 0.2-0.4 ng/ml IL-5 or 3-6 ng/ml IL-13, the expression of the MMP-2 and -9 in the CRS cultures was analysed. Results: After 72 hours incubation with IL-5, the relative levels of MMP-2 showed no significant alteration in protein expression in comparison with the control groups. Incubation with IL-13 revealed a statistically insignificant decrease of the relative MMP-9 expression in ECRS compared to the control group (p>0.1). Conclusion: Alterations of MMP-2 and -9 expression may play a role in ECRS, but the association with IL-5 and IL-13 remains unclear.
|Item Type:||Journal Article|
|Divisions:||Faculty of Medicine > Warwick Medical School|
|Journal or Publication Title:||In Vivo|
|Publisher:||International Institute of Anticancer Research|
|Number of Pages:||7|
|Page Range:||pp. 415-421|
|Access rights to Published version:||Restricted or Subscription Access|
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