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Hairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporus
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Eastwood, Daniel C., Challen, Michael P., Zhang, Cunjin, Jenkins, H. (Huw), Henderson, Janey and Burton, Kerry S. (2008) Hairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporus. Mycological Research, Vol.112 (Part 6). pp. 708-716. doi:10.1016/j.mycres.2008.01.009 ISSN 0953-7562.
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Official URL: http://dx.doi.org/10.1016/j.mycres.2008.01.009
Abstract
A double-stranded (ds) RNA hairpin-mediated down-regulation system was developed for the cultivated mushroom Agaricus bisporus, and the role of the urea cycle enzyme argininosuccinate lyase (asl) in mushroom post-harvest development was investigated. Hairpin expression vectors were constructed to initiate down-regulation of as] and introduced into A. bisporus by Agrobacterium tumefaciens-mediated transformation. Transcripts of asl were significantly reduced (93.1 and 99.9%) in two transformants and hairpin vector transgene sequences were maintained throughout sporophore development. Single and multiple hairpin integration events were observed in Southern analysis. Transformants with down-regulated asl exhibited reduced yield and cap expansion during post-harvest sporophore development. There were no detectable differences in urea levels between the hairpin-transformed and control strains. This is the first report of reduced gene expression resulting from the introduction of dsRNA hairpins in A. bisporus and the applications of this technology will facilitate functional studies in the mushroom. (C) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QK Botany Q Science > QP Physiology S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
| Library of Congress Subject Headings (LCSH): | Cultivated mushroom, Agrobacterium, Enzymes, RNA, Urea | ||||
| Journal or Publication Title: | Mycological Research | ||||
| Publisher: | Cambridge University Press | ||||
| ISSN: | 0953-7562 | ||||
| Official Date: | June 2008 | ||||
| Dates: |
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| Volume: | Vol.112 | ||||
| Number: | Part 6 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 708-716 | ||||
| DOI: | 10.1016/j.mycres.2008.01.009 | ||||
| Status: | Not Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA) | ||||
| Grant number: | HH2116SMU (DEFRA) |
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