Eastwood, Daniel C., Challen, Michael P., Zhang, Cunjin, Jenkins, H. (Huw), Henderson, Janey and Burton, Kerry S.. (2008) Hairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporus. Mycological Research, Vol.112 (Part 6). pp. 708-716. ISSN 0953-7562

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Abstract

A double-stranded (ds) RNA hairpin-mediated down-regulation system was developed for the cultivated mushroom Agaricus bisporus, and the role of the urea cycle enzyme argininosuccinate lyase (asl) in mushroom post-harvest development was investigated. Hairpin expression vectors were constructed to initiate down-regulation of as] and introduced into A. bisporus by Agrobacterium tumefaciens-mediated transformation. Transcripts of asl were significantly reduced (93.1 and 99.9%) in two transformants and hairpin vector transgene sequences were maintained throughout sporophore development. Single and multiple hairpin integration events were observed in Southern analysis. Transformants with down-regulated asl exhibited reduced yield and cap expansion during post-harvest sporophore development. There were no detectable differences in urea levels between the hairpin-transformed and control strains. This is the first report of reduced gene expression resulting from the introduction of dsRNA hairpins in A. bisporus and the applications of this technology will facilitate functional studies in the mushroom. (C) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany Q Science > QP Physiology S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Cultivated mushroom, Agrobacterium, Enzymes, RNA, Urea
Journal or Publication Title:	Mycological Research
Publisher:	Cambridge University Press
ISSN:	0953-7562
Date:	June 2008
Volume:	Vol.112
Number:	Part 6
Number of Pages:	9
Page Range:	pp. 708-716
Identification Number:	10.1016/j.mycres.2008.01.009
Status:	Not Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA)
Grant number:	HH2116SMU (DEFRA)
References:	Beecher TM, Magan N, Burton KS, 2001. Water potentials and soluble carbohydrate concentrations in tissues of freshly harvested and stored mushrooms (Agaricus bisporus). Post harvest Biology and Technology 22: 121–131. Braaksma A, van Doorn AA, Kieft H, van Aelst AC, 1998. Morphogenic analysis of ageing mushrooms (Agaricus bisporus) during post-harvest development. Post harvest Biology and Technology 13: 71–79. Burns C, Gregory KE, Kirby M, Cheung MK, Riquelme M, Elliot TJ, Challen MP, Bailey A, Foster GD, 2005. Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns. Fungal Genetics and Biology 42: 191–199. Burton KS, 2004. Cultural factors affecting mushroom quality d cause and control of bruising. Mushroom Science 16: 397–402. Burton KS, Partis MD, Wood DA, Thurston CF, 1997. Accumulation of serine proteinase in senescent sporophores of the cultivated mushroom, Agaricus bisporus. Mycological Research 101: 146–152. Chen X, Stone M, Schlagnhaufer C, Romaine CP, 2000. A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus. Applied Environmental Microbiology 66: 4510–4513. De Windt FE, Wagemaker MJM, Op den Camp HJM, van der Drift C, 2002. Purine degradation in the edible mushroom Agaricus bisporus. Folia Microbiologica 47: 672–676. Donker HCW, van As H, 1999. Cell water balance of white button mushrooms (Agaricus bisporus) during its post-harvest lifetime studied by quantitative magnetic resonance imaging. Biochimica et Biophysica Acta 1427: 287–297. Eastwood DC, Kingsnorth CS, Jones HE, Burton KS, 2001. Genes with increased transcript levels following harvest of the sporophore of Agaricus bisporus have multiple physiological roles. Mycological Research 105: 1223–1230. Elliott TJ, 1985a. The genetics and breeding of species of Agaricus. In: Flegg PB, Spencer DM, Wood DA (eds), The Biology and Technology of the Cultivated Mushroom. John Wiley & Sons, Chichester, pp. 111–129. Elliott TJ, 1985b. Spawn-making and spawns. In: Flegg PB, Spencer DM, Wood DA (eds), The Biology and Technology of the Cultivated Mushroom. John Wiley & Sons, Chichester, pp. 131–139. Ewaze JO, Moore D, Stewart GR, 1979. Co-ordinate regulation of enzymes involved in ornithine metabolism and its relation to sporophore morphogenesis in Coprinus cinereus. Journal of General Microbiology 107: 343–357. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC, 1998. Potent and specific genetic interference by doublestranded RNA in Caenorhabditis elegans. Nature 391: 806–811. Forrest EC, Cogoni C, Macino G, 2004. The RNA-dependant RNA polymerase, QDE-1, is a rate-limiting factor in posttranscriptional gene silencing in Neurospora crassa. Nucleic Acids Res 32: 2123–2128. Goidin D, Mamessier A, Statquet M-J, Schmitt D, Berthier- Vergnes O, 2000. Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and b-actin genes as internal standards for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations. Annals of Biochemistry 295: 17–21. Goldoni M, Azzalin G, Macino G, Cogoni C, 2004. Efficient gene silencing by expression of double stranded RNA in Neurospora crassa. Fungal Genetics and Biology 41: 1016–1024. de Groot MJA, Bundock P, Hoogkaas PJJ, Beijersbergen AGM, 1998. Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nature Biotechnology 16: 839–842. Hammond JBW, 1979. Changes in composition of harvested mushrooms (Agaricus bisporus). Phytochemistry 18: 415–418. Hammond JBW, Nichols R, 1975. Changes in respiration and soluble carbohydrates during post-harvest storage of mushrooms (Agaricus bisporus). Journal of the Science of Food and Agriculture 26: 835–842. Hammond JBW, Nichols R, 1976. Carbohydrate metabolism in Agaricus bisporus (Lange) Sign.: changes in soluble carbohydrates during growth of mycelium and fruiting body. Journal of General Microbiology 93: 309–320. Heneghan MN, Costa AMSB, Challen MP, Mills PR, Bailey A, Foster GD, 2007. A comparison of methods for successful triggering of gene silencing in Coprinus cinereus. Molecular Biotechnology 35: 283–298. Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM, 2000a. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Molecular Biology 42: 819–832. Hellens RP, Mullineaux P, Klee H, 2000b. A guide to Agrobacterium binary Ti vectors. Trends in Plant Science 5: 446–451. de Jong JF, Deelstra HJ, Wosten HAB, Lugones LG, 2006. RNAmediated gene silencing in monokaryons and dikaryons of Schizophyllum commune. Applied Environmental Microbiology 72: 1267–1269. Kingsnorth CS, Eastwood DC, Burton KS, 2001. Cloning and postharvest expression of serine proteinase transcripts in the cultivated mushroom Agaricus bisporus. Fungal Genetics and Biology 32: 135–144. Lamontagne B, Hannoush RN, Damha MJ, Abou Elela S, 2004. Molecular requirements for duplex recognition and cleavage by eukaryotic RNase III: discovery of an RNA-dependent DNA cleavage activity of yeast Rnt1p. Journal of Molecular Biology 338: 401–418. Leach K, Odon V, Zhang C, Kim HK, Henderson J, Warner P, Challen M, Elliott T, 2004. Progress in Agaricus bisporus transformation: Agrobacterium methodologies and development of novel marker genes. Mushroom Science 16: 93–102. Liu H, Cotterell TR, Pierini LM, Goldman WE, Doering TL, 2002. RNA interference in the pathogenic fungus Cryptococcus noeformans. Genetics 160: 463–470. McGarry A, Burton KS, 1994. Mechanical-properties of the mushroom, Agaricus bisporus. Mycological Research 98: 241–245. Mikosch TSP, Lavrijssen B, Sonnenberg ASM, van Griensven LJLD, 2001. Transformation of the cultivated mushroom Agaricus bisporus (Lange) using T-DNA from Agrobacterium tumefaciens. Current Genetics 39: 35–39. Mills PM, Thomas JD, Sergeant MJ, Costa A, Collopy P, Bailey A, Foster G, Challen MP, 2007. Interactions between Agaricus bisporus and the pathogen Verticillium fungicola. In: Avery S, van West P (eds), Stress in Yeasts and Filamentous Fungi. Proceedings of the British Mycological Society Annual Scientific Meeting, 4–7 September 2006. University of Birmingham. Moore D, 1985. Developmental biology of the Coprinus cinereus carpophore: metabolic regulation in relation to cap morphogenesis. Experimental Mycology 8: 283–297. Nakayashiki H, 2005. RNA silencing in fungi: mechanisms and applications. FEBS Letters 579: 5950–5957. Nakayashiki H, Hanada S, Bao Quoc N, Kadotani N, Tosa Y, Mayama S, 2004. RNA silencing as a tool for exploring gene function in ascomycete fungi. Fungal Genetics and Biology 42: 275–283. Nakayashiki H, Kadotani N, Mayama S, 2006. Evolution and diversification of RNA silencing proteins in fungi. Journal of Molecular Evolution 63: 127–135. Namekawa SH, Iwabata K, Sugawara H, Hamada FN, Koshiyama A, Chiku H, Kamada T, Sakaguchi K, 2005. Knockdown of LIM15/DMC1 in the mushroom Coprinus cinereus by double-stranded RNA-mediated gene silencing. Microbiology SGM 151: 3669–3679. Nolan T, Braccini L, Azzalin G, de Toni A, Macino G, Cogoni C, 2005. The post-transcriptional gene silencing machinery functions independantly of DNA methylation to repress a LINE1-like retrotransposon in Neurospora crassa. Nucleic Acids Research 33: 1564–1573. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ, 1987. Transformation of Aspergillus based on the hygromycing-B resistance gene from Escherichia coli. Gene 56: 117–124. Rosen KM, Villa-Kormaroff L, 1993. An alternative method for the visualisation of RNA in formaldehyde agarose gels. Focus 12: 23–24. Sugui JA, Chang YC, Kwon-Chung KJ, 2005. Agrobacterium tumefaciens- mediated transformation of Aspergillus fumigatus: an efficient tool for insertional mutagenesis and targeted gene disruption. Applied Environmental Microbiology 71: 1789–1802. Wagemaker MJM, Eastwood DC, van der Drift C, Jetten MSM, Burton KS, van Griensven LJLD, Op den Camp HJM, 2006. Expression of the urease gene of Agaricus bisporus: a tool for studying fruit body formation and post-harvest development. Applied Microbiology and Biotechnology 71: 486–492. Wagemaker MJM, Eastwood DC, van der Drift C, Jetten MSM, Burton KS, van Griensven LJLD, Op den Camp HJM, 2007a. Argininosuccinate synthetase and argininosuccinate lyase: two ornithine cycle enzymes from Agaricus bisporus. Mycological Research 111: 493–502. Wagemaker MJM, Eastwood DC, van der Drift C, Jetten MSM, Burton KS, van Griensven LJLD, Op den Camp HJM, 2007b. The role of ornithine aminotransferase in fruiting body formation of the mushroom Agaricus bisporus. Mycological Research 111: 909–918. Wa¨ lti MA, Villalba C, Buser RM, Gru¨ nler A, Aebi M, Ku¨ nzler M, 2006. Targetted gene silencing in the model mushroom Coprinopsis cinerea (Coprinus cinereus) by expression of homologous hairpin RNAs. Eukaryotic Cell 5: 732–744.
URI:	http://wrap.warwick.ac.uk/id/eprint/29707

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