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Surfactant protein a binds to HIV and inhibits direct infection of CD4(+) cells, but enhances dendritic cell-mediated viral transfer
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Gaiha, Gaurav D., Dong, Tao, Palaniyar, Nades, Mitchell, Daniel Anthony, Reid, Kenneth B. M. and Clark, Howard W.. (2008) Surfactant protein a binds to HIV and inhibits direct infection of CD4(+) cells, but enhances dendritic cell-mediated viral transfer. Journal of Immunology, Vol.181 (No.1). pp. 601-609. ISSN 0022-1767
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Official URL: http://www.jimmunol.org/content/181/1/601.abstract
Abstract
The identification of surfactant protein A (SP-A) as an important innate immune factor of the lungs, amniotic fluid, and the vaginal tract suggests that it could play an important role during various stages of HIV disease progression and transmission. Therefore, we examined whether SP-A could bind to HIV and also had any effect on viral infectivity. Our data demonstrate that SP-A binds to HIV in a calcium-dependent manner that is inhibitable by mannose and EDTA. Affinity capture of the HIV viral lysate reveals that SP-A targets the envelope glycoprotein of HIV (gp120), which was confirmed by ELISA using recombinant gp120. Digestion of gp120 with endoglycosidase H abrogates the binding of SP-A, indicating that the high mannose structures on gp120 are the target of the collectin. Infectivity studies reveal that SP-A inhibits the infection of CD4(+) T cells by two strains of HIV (BaL, IIIB) by >80%. Competition assays with CD4 and mAbs F105 and b12 suggest that SP-A inhibits infectivity by occlusion of the CD4-binding site. Studies with dendritic cells (DCs) demonstrate that SP-A enhances the binding of gp120 to DCs, the uptake of viral particles, and the transfer of virus from DCs to CD4+ T cells by >5-fold at a pH representative of the vaginal tract. Collectively, these results suggest that SP-A acts as a dual modulator of HIV infection by protecting CD4+ T cells from direct infection but enhancing the transfer of infection to CD4+ T cells mediated by DCs.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology > QR180 Immunology |
| Divisions: | Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School |
| Journal or Publication Title: | Journal of Immunology |
| Publisher: | American Association of Immunologists |
| ISSN: | 0022-1767 |
| Date: | 1 July 2008 |
| Volume: | Vol.181 |
| Number: | No.1 |
| Number of Pages: | 9 |
| Page Range: | pp. 601-609 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/29755 |
Data sourced from Thomson Reuters' Web of Knowledge
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