A proteomic approach to the identification of the major virion structural proteins of the marine cyanomyovirus S-PM2
Clokie, Martha R. J., Thalassinos, Konstantinos, Boulanger, Pascale, Slade, Susan E., Stoilova-McPhie, Svetla, Cane, Matt, Scrivens, James H. and Mann, Nicholas H.. (2008) A proteomic approach to the identification of the major virion structural proteins of the marine cyanomyovirus S-PM2. Microbiology, Vol.154 (Part 6). pp. 1775-1782. ISSN 1350-0872Full text not available from this repository.
Official URL: http://dx.doi.org/10.1099/mic.0.2007/016261-0
In this study, an MS-based proteomics approach to characterizing the virion structural proteins of the novel marine 'photosynthetic' phage S-PM2 is presented. The virus infects ecologically important cyanobacteria of the genus Synechococcus that make a substantial contribution to primary production in the oceans. The S-PM2 genome encodes 236 ORFs, some of which exhibit similarity to known phage virion structural proteins, but the majority (54%) show no detectable homology to known proteins from other organisms. Using public and in-house bioinformatics; tools the proteome of S-PM2 was predicted and a database compatible with MS-based search engines was constructed. S-PM2 virion proteins were resolved by SIDS-PAGE, excised, tryptically digested and analysed by LC-ESI-MS/MS. The resulting MS data were searched against the database. A parallel control study was undertaken on the well-characterized coliphage T4 in order to assess the sensitivity and efficiency of this approach. In total, 11 of the 15 S-PM2 proteins, predicted to be virion proteins by bioinformatics approaches, were confirmed as such, together with the identification of a further 12 novel structural proteins. In the case of T4, 24 of the 39 known virion structural proteins were identified, including the major tail-fibre proteins. This approach has wide-ranging applicability and can be applied to any novel organism whose genome encodes ORFs with few detectable homologies in the public databases.
|Item Type:||Journal Article|
|Subjects:||Q Science > QP Physiology
Q Science > QR Microbiology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Library of Congress Subject Headings (LCSH):||Proteomics, Viral proteins, Bacteriophages, Marine microbiology|
|Journal or Publication Title:||Microbiology|
|Publisher:||Society for General Microbiology|
|Official Date:||June 2008|
|Number of Pages:||8|
|Page Range:||pp. 1775-1782|
|Access rights to Published version:||Restricted or Subscription Access|
Akhter, T., Zhao, L., Kohda, A., Mio, K., Kanamaru, S. & Arisaka, F.
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