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Minimally invasive spatial and temporal concentration profiling within working glucose biosensors

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Burt, David P. and Unwin, Patrick R. (2008) Minimally invasive spatial and temporal concentration profiling within working glucose biosensors. Electrochemistry Communications, Vol.10 (No.6). pp. 934-937. doi:10.1016/j.elecom.2008.04.019

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Official URL: http://dx.doi.org/10.1016/j.elecom.2008.04.019

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Abstract

The time-dependent evolution of analyte during the operation of working glucose biosensors has been profiled using a carbon fibre microelectrode (CFE). A simple procedure for the fabrication of minimally invasive CFE probes (<8 mu m maximum diameter) is described. By positioning the CFE at well-defined distances from a working biosensor surface it has been possible to track the concentration of ferrocyanide, generated in the enzymatic assay for glucose, as a function of space and time. The results indicate that on the timescale of operation of typical glucose biosensors, the enzyme-mediated oxidation of glucose only occurs to a significant extent in a thin layer close to the sensor surface. In much of the analyte volume the reaction is negligible, contrary to present consensus. (C) 2008 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Library of Congress Subject Headings (LCSH): Biosensors, Gluscose, Ultramicroelectrodes, Scanning electrochemical microscopy
Journal or Publication Title: Electrochemistry Communications
Publisher: Elsevier Inc.
ISSN: 1388-2481
Official Date: June 2008
Dates:
DateEvent
June 2008Published
Volume: Vol.10
Number: No.6
Number of Pages: 4
Page Range: pp. 934-937
DOI: 10.1016/j.elecom.2008.04.019
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

Data sourced from Thomson Reuters' Web of Knowledge

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