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Functional characterization of PaLAX1, a putative auxin permease, in heterologous plant systems
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Hoyerova, Klara, Perry, Lucie, Hand, Paul, Lankova, Martina, Kocabek, Tomas, May, Sean T., Kottova, Jana, Paces, Jan, Napier, R. (Richard) and Zazimalova, Eva (2008) Functional characterization of PaLAX1, a putative auxin permease, in heterologous plant systems. Plant Physiology, Volume 146 (Number 3). pp. 1128-1141. doi:10.1104/pp.107.109371 ISSN 0032-0889.
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WRAP_Napier_0380026-lf-220814-klara_and_lucie_paper_plant_phys_jan_2008.pdf - Accepted Version - Requires a PDF viewer. Download (1441Kb) | Preview |
Official URL: http://dx.doi.org/10.1104/pp.107.109371
Abstract
We have isolated the cDNA of the gene PaLAX1 from a wild cherry tree ( Prunus avium). The gene and its product are highly similar in sequences to both the cDNAs and the corresponding protein products of AUX/LAX-type genes, coding for putative auxin influx carriers. We have prepared and characterized transformed Nicotiana tabacum and Arabidopsis thaliana plants carrying the gene PaLAX1. We have proved that constitutive overexpression of PaLAX1 is accompanied by changes in the content and distribution of free indole-3-acetic acid, the major endogenous auxin. The increase in free indole-3-acetic acid content in transgenic plants resulted in various phenotype changes, typical for the auxin-overproducing plants. The uptake of synthetic auxin, 2,4-dichlorophenoxyacetic acid, was 3 times higher in transgenic lines compared to the wild-type lines and the treatment with the auxin uptake inhibitor 1-naphthoxyacetic acid reverted the changes caused by the expression of PaLAX1. Moreover, the agravitropic response could be restored by expression of PaLAX1 in the mutant aux1 plants, which are deficient in auxin influx carrier activity. Based on our data, we have concluded that the product of the gene PaLAX1 promotes the uptake of auxin into cells, and, as a putative auxin influx carrier, it affects the content and distribution of free endogenous auxin in transgenic plants.
Item Type: | Journal Article | ||||
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Subjects: | S Agriculture > SB Plant culture | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
Library of Congress Subject Headings (LCSH): | Sweet cherry -- Genetics, Tobacco -- Genetics, Arabidopsis thaliana -- Genetics, Auxin, Transgenic plants | ||||
Journal or Publication Title: | Plant Physiology | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 0032-0889 | ||||
Official Date: | March 2008 | ||||
Dates: |
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Volume: | Volume 146 | ||||
Number: | Number 3 | ||||
Number of Pages: | 14 | ||||
Page Range: | pp. 1128-1141 | ||||
DOI: | 10.1104/pp.107.109371 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 14 December 2015 | ||||
Date of first compliant Open Access: | 14 December 2015 | ||||
Funder: | Czech Republic, Akademie věd České republiky [Academy of Sciences of the Czech Republic], Czech Republic. Ministerstvo školství, mládeže a tělovýchovy [Ministry of Education, Youth, and Sport], Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | 206/02/P106 (CR), 206/02/0967 (CR), B6038203 (ASCR), A6038303 (ASCR), KJB600380702 (ASCR), LN06034 (MEYS), 1M0520 (MEYS), AV0Z50380511 (CR), AV0Z50510513 (CR) |
Data sourced from Thomson Reuters' Web of Knowledge
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