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Tumor risk assessment by means of immunocytochemical detection of early pre-malignant changes in buccal smears
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Bloching, Marc M., Soulsby, Hannah, Naumann, Andreas, Aust, Wolfram, Merkel, Daniel, Braun, Theresa and Naim, Ramin. (2008) Tumor risk assessment by means of immunocytochemical detection of early pre-malignant changes in buccal smears. Oncology Reports, Vol.19 (No.6). pp. 1373-1379. ISSN 1021-335X
Full text not available from this repository.Abstract
Increasing incidence of carcinomas in the upper aero-digestive tract, both in Germany and in other European countries requires development of new preventive strategies. The cure rate at advanced tumor stages remains poor in spite of a variety of available therapeutic methods. In the present study the quantitative assessment of a pre-malignant mucosa lesion within a field cancerization was performed by means of immunocytochemical methods. This may allow individuals with an increased risk of developing malignant disease to be identified. Cytosmears taken from healthy buccal mucosa of tumor patients (n=50) and from healthy probands (n=100) with different tobacco and alcohol consumption levels were examined with regard to identifying increased expression of the proliferation markers (PCNA, MIB1), of the tumor suppressor gene product p53 as well as the oncogene product cyclin D1. There was a significant difference in expression of investigated proliferation markers between tumor patients and healthy probands (p<0.0001). When comparing the rate of positively marked cell nuclei to cigarette pack years the marker cyclin D1 and MIB1 show an increased rate in the groups with high tobacco consumption as compared to the group with a low exposure (p>0.05). It could be possible to use the marker MIBI and cyclin D1 to screen risk groups, since the relative morbidity risk (odds ratio) increases (by 45-62 times) if the threshold value of 4 positively marked cell nuclei is exceeded.
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine > RA Public aspects of medicine R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| Divisions: | Faculty of Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Tumors -- Risk factors, Tumors -- Diagnosis, Medical screening, Carcinogenesis, Immunocytochemistry, Cancer -- Prevention, Squamous cell carcinoma |
| Journal or Publication Title: | Oncology Reports |
| Publisher: | Spandidos Publications |
| ISSN: | 1021-335X |
| Date: | June 2008 |
| Volume: | Vol.19 |
| Number: | No.6 |
| Number of Pages: | 7 |
| Page Range: | pp. 1373-1379 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Related URLs: | |
| References: | 1. Boyle P, Macfarlane GJ, Blot WJ, Chiesa F, Lefebvre JL, Azul AM, De Vries N and Scully C: European School of Oncology Advisory report to the European Commission for the Europe Against Cancer Programme: oral carcinogenesis in Europe. Eur J Cancer B Oral Oncol 31: 75-85, 1995. 2. Haas JF, Rahu M and Staneczek W: Time trends in cancer incidence in the German Democratic Republic 1968-1981. Neoplasma 33: 129-139, 1986. 3. La Vecchia C, Tavani A, Franceschi S, Levi F, Corrao G and Negri E: Epidemiology and prevention of oral cancer. Oral Oncol 33: 302-312, 1997. 4. Bootz F: Reports from the Aachen annual meeting of the German Society for ENT Medicine, Head and Neck Surgery. Oncology- Ear Surgery. HNO 47: 859-862, 1999. 5. Ambrosch P: Screening studies for early detection of carcinomas of the upper aerodigestive tract. HNO 44: 609-611, 1996. 6. Slaughter DP, Southwick HW and Smejkal W: Field cancerization in oral stratified squamous epithelium; clinical implications of multicentric origin. Cancer 6: 963-968, 1953. 7. Weinberg RA: How cancer arises. Sci Am 275: 62-70, 1996. 8. Carrano AV and Natarajan AT: International Commission for Protection Against Environmental Mutagens and Carcinogens. ICPEMC publication no. 14. Considerations for population monitoring using cytogenetic techniques. Mutat Res 204: 379-406, 1988. 9. Anders A, Groger H, Anders F, Zechel C, Smith A and Schlatterer B: Discrimination of initiating and promoting carcinogens in fish. Ann Rech Vet 22: 273-294, 1991. 10. Anders A, Zechel C, Schlatterer B, Groger H, Schmidt D, Smith A and Anders F: Genetic and molecular approach for breeding and use of laboratory fish for the detection of agents with carcinogenic and/or promoting activity. Bull Cancer 78: 415-433, 1991. 11. Cohen SM and Ellwein LB: Genetic errors, cell proliferation, and carcinogenesis. Cancer Res 51: 6493-6505, 1991. 12. Renan MJ: How many mutations are required for tumorigenesis? Implications from human cancer data. Mol Carcinog 7: 139-146, 1993. 13. Califano J, van der Riet P, Westra W, Nawroz H, Clayman G, Piantadosi S, Corio R, Lee D, Greenberg B, Koch W and Sidransky D: Genetic progression model for head and neck cancer: implications for field cancerization. Cancer Res 56: 2488-2492, 1996. 14. Bradford CR, Zhu S, Poore J, Fisher SG, Beals TF, Thoraval D, Hanash SM, Carey TE and Wolf GT: p53 mutation as a prognostic marker in advanced laryngeal carcinoma. Department of Veterans Affairs Laryngeal Cancer Cooperative Study Group. Arch Otolaryngol Head Neck Surg 123: 605-609, 1997. 15. Masuda M, Hirakawa N, Nakashima T, Kuratomi Y and Komiyama S: Cyclin D1 overexpression in primary hypopharyngeal carcinomas. Cancer 78: 390-395, 1996. 16. Mineta H, Borg A, Dictor M, Wahlberg P and Wennerberg J: Correlation between p53 mutation and cyclin D1 amplification in had and neck squamous cell carcinoma. Oral Oncol 33: 42-46, 1997. 17. Mineta H, Miura K, Takebayashi S, Ueda Y, Misawa K, Harada H, Wennerberg J and Dictor M: Cyclin D1 overexpression correlates with poor prognosis in patients with tongue squamous cell carcinoma. Oral Oncol 36: 194-198, 2000. 18. Cohen SM and Ellwein LB: Cell proliferation in carcinogenesis. Science 249: 1007-1011, 1990. 19. Shin DM, Voravud N, Ro JY, Lee JS, Hong WK and Hittelman WN: Sequential increases in proliferating cell nuclear antigen expression in head and neck tumorigenesis: a potential biomarker. J Natl Cancer Inst 85: 971-978, 1993. 20. Schwartz JL, Gu X, Kittles RA, Baptiste A and Shklar G: Experimental oral carcinoma of the tongue and buccal mucosa: possible biologic markers linked to cancers at two anatomic sites. Oral Oncol 36: 225-235, 2000. 21. Bloching M, Hofmann A, Lautenschlager C, Berghaus A and Grummt T: Exfoliative cytology of normal buccal mucosa to predict the relative risk of cancer in the upper aerodigestive tract using the MN-assay. Oral Oncol 36: 550-555, 2000. 22. Piffko J, Bankfalvi A, Joos U, Ofner D, Krassort M and Schmid KW: Immunophenotypic analysis of normal mucosa and squamous cell carcinoma of the oral cavity. Cancer Detect Prev 23: 45-56, 1999. 23. Liu SC and Klein-Szanto AJ: Markers of proliferation in normal and leukoplakic oral epithelia. Oral Oncol 36: 145-151, 2000. 24. Martinez-Lara I, Gonzalez-Moles MA, Ruiz-Avila I, Bravo M, Ramos MC and Fernandez-Martinez JA: Proliferating cell nuclear antigen (PCNA) as a marker of dysplasia in oral mucosa. Acta Stomatol Belg 93: 29-32, 1996. 25. Zain RB, Sakamoto F, Shrestha P and Mori M: Proliferating cell nuclear antigen (PCNA) expression in oral squamous cell carcinoma - an aid to conventional histological grading? Malays J Pathol 17: 23-30, 1995. 26. Prives C and Hall PA: The p53 pathway. J Pathol 187: 112-126, 1999. 27. Bosch FX, Homann N, Conradt C, Dietz A and Erber R: p53 mutations/p53 protein overexpression. Differential significance for the progression of head-neck carcinomas. HNO 47: 833-848, 1999. 28. Ahomadegbe JC, Barrois M, Fogel S, Le Bihan ML, Douc- Rasy S, Duvillard P, Armand JP and Riou G: High incidence of p53 alterations (mutation, deletion, overexpression) in head and neck primary tumors and metastases; absence of correlation with clinical outcome. Frequent protein overexpression in normal epithelium and in early non-invasive lesions. Oncogene 10: 1217-1227, 1995. 29. Ma L, Ronai A, Riede UN and Kohler G: Clinical implication of screening p53 gene mutations in head and neck squamous cell carcinomas. J Cancer Res Clin Oncol 124: 389-396, 1998. 30. Kyomoto R, Kumazawa H, Toda Y, Sakaida N, Okamura A, Iwanaga M, Shintaku M, Yamashita T, Hiai H and Fukumoto M: Cyclin-D1-gene amplification is a more potent prognostic factor than its protein over-expression in human head-and-neck squamous-cell carcinoma. Int J Cancer 74: 576-581, 1997. 31. Bartkova J, Lukas J, Muller H, Strauss M, Gusterson B and Bartek J: Abnormal patterns of D-type cyclin expression and G1 regulation in human head and neck cancer. Cancer Res 55: 949-956, 1995. 32. Friedberg EC, Fuchs RP, Goodman MF, Hanaoka F, Hinkle D, Kunkel TA, Lawrence CW, Livneh Z, Nohmi T, Prakash L, Prakash S, Todo T, Walker GC, Wang Z and Woodgate R: The Y-family of DNA polymerases. Mol Cell 8: 7-8, 2001. 33. Lindahl T, Sedgwick B, Sekiguchi M and Nakabeppu Y: Regulation and expression of the adaptive response to alkylating agents. Annu Rev Biochem 57: 133-157, 1988. 34. Simic MG: Introduction to mechanisms of DNA damage and repair. Basic Life Sci 38: 1-8, 1986. |
| URI: | http://wrap.warwick.ac.uk/id/eprint/29981 |
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