Hamberger, Alexandra, Horn, Marcus A., Dumont, Marc G., Murrell, J. C. (J. Colin) and Drake, Harold L., 1952-. (2008) Anaerobic consumers of monosaccharides in a moderately acidic fen. Applied and Environmental Microbiology, Vol.74 (No.10). pp. 3112-3120. ISSN 0099-2240

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Abstract

16S rRNA-based stable isotope probing identified active xylose- and glucose-fermenting Bacteria and active Archaea, including methanogens, in anoxic slurries of material obtained from a moderately acidic, CH4 emitting fen. Xylose and glucose were converted to fatty acids, CO2, H-2, and CH4 under moderately acidic, anoxic conditions, indicating that the fen harbors moderately acid-tolerant xylose- and glucose-using fermenters, as well as moderately acid-tolerant methanogens. Organisms of the families Acidaminococcaceae, Aeromonadaceae, Clostridiaceae, Enterobacteriaceae, and Pseudomonadaceae and the order Actinomycetales, including hitherto unknown organisms, utilized xylose- or glucose-derived carbon, suggesting that highly diverse facultative aerobes and obligate anaerobes contribute to the flow of carbon in the fen under anoxic conditions. Uncultured Euryarchaeota (i.e., Methanosarcinaceae and Methanobacteriaceae) and Crenarchaeota species were identified by 16S rRNA analysis of anoxic slurries, demonstrating that the acidic fen harbors novel methanogens and Crenarchaeota organisms capable of anaerobiosis. Fermentation-derived molecules are conceived to be the primary drivers of methanogenesis when electron acceptors other than CO2 are absent, and the collective findings of this study indicate that fen soils harbor diverse, acid-tolerant, and novel xylose-utilizing as well as glucose-utilizing facultative aerobes and obligate anaerobes that form trophic links to novel moderately acid-tolerant methanogens.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Anaerobic bacteria, Monosaccharides, Acid soils, Fens, Fen ecology
Journal or Publication Title:	Applied and Environmental Microbiology
Publisher:	American Society for Microbiology
ISSN:	0099-2240
Date:	May 2008
Volume:	Vol.74
Number:	No.10
Number of Pages:	9
Page Range:	pp. 3112-3120
Identification Number:	10.1128/AEM.00193-08
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Deutsche Forschungsgemeinschaft (DFG), Universität Bayreuth
Grant number:	Dr310/3-2 (DFG)
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URI:	http://wrap.warwick.ac.uk/id/eprint/30002

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