Lalo, Ulyana, Pankratov, Yuriy, Wichert, Sven P., Rossner, Moritz J., North, R. Alan, Kirchhoff, Frank and Verkhratsky, A. N. (Alekseĭ Nestorovich). (2008) P2X(1) and P2X(5) Subunits form the functional P2X receptor in mouse cortical astrocytes. Journal of Neuroscience, Vol.28 (No.21). pp. 5473-5480. ISSN 0270-6474

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Abstract

ATP plays an important role in signal transduction between neuronal and glial circuits and within glial networks. Here we describe currents activated by ATP in astrocytes acutely isolated from cortical brain slices by non-enzymatic mechanical dissociation. Brain slices were prepared from transgenic mice that express enhanced green fluorescent protein under the control of the human glial fibrillary acidic protein promoter. Astrocytes were studied by whole-cell voltage clamp. Exogenous ATP evoked inward currents in 75 of 81 astrocytes. In the majority (similar to 65%) of cells, ATP-induced responses comprising a fast and delayed component; in the remaining subpopulation of astrocytes, ATP triggered a smoother response with rapid peak and slowly decaying plateau phase. The fast component of the response was sensitive to low concentrations of ATP (with EC50 of similar to 40 nM). All ATP-induced currents were blocked by pyridoxal-phosphate-6-azophenyl- 2',4'- disulfonate (PPADS); they were insensitive to ivermectin. Quantitative real-time PCR demonstrated strong expression of P2X(1) and P2X(5) receptor subunits and some expression of P2X(2) subunit mRNAs. The main properties of the ATP-induced response in cortical astrocytes (high sensitivity to ATP, biphasic kinetics, and sensitivity to PPADS) were very similar to those reported for P2X(1/5) heteromeric receptors studied previously in heterologous expression systems.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Purines -- Receptors, Adenosine triphosphate, Cellular signal transduction, Astrocytes, Transgenic mice, Neural transmission
Journal or Publication Title:	Journal of Neuroscience
Publisher:	Society for Neuroscience
ISSN:	0270-6474
Date:	21 May 2008
Volume:	Vol.28
Number:	No.21
Number of Pages:	8
Page Range:	pp. 5473-5480
Identification Number:	10.1523/JNEUROSCI.1149-08.2008
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Wellcome Trust (London, England), National Institutes of Health (U.S.) (NIH), Alzheimer Research Trust, Deutsche Forschungsgemeinschaft (DFG), Max-Planck-Gesellschaft zur Förderung der Wissenschaften [Max Planck Society for the Advancement of Science]
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URI:	http://wrap.warwick.ac.uk/id/eprint/30019

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