Lin, Y. -C., Miles, Roger (Roger J.), Nicholas, R. A. J., Kelly, Donovan P., 1940- and Wood, Ann P.. (2008) Isolation and immunological detection of Mycoplasma ovipneumoniae in sheep with atypical pneumonia, and lack of a role for Mycoplasma arginini. Research in Veterinary Science, Vol.84 (No.3). pp. 367-373. ISSN 0034-5288

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Abstract

Mycoplasma ovipneumoniae NCTC 10151(T) and four new isolates from UK sheep flocks were compared. Only glucose and pyruvate were used as energy sources by the five strains: glucose was the best energy source for the type strain, pyruvate supported better growth of the new strains. Whole cell protein patterns and antigenic profiles showed high similarity between all five strains. The new isolates fell into two groups in ELISA tests. Serum samples from 30 pneumonic sheep were assessed for M. ovipneumoniae infection and Mycoplasma arginini co-infection. Fourteen (out of 30) serum samples were positive for M. ovipneumoniae both by ELISA and immunoblotting. Twelve antigenic proteins of M. ovipneumoniae were detected in infected serum samples: the antigen patterns were unique, with between one and at least seven occurring in any one sample. All serum samples were designated as negative for M. arginini antibodies by both ELISA and immunoblotting. (c) 2007 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology Q Science > QR Microbiology > QR180 Immunology S Agriculture > SF Animal culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Mycoplasma diseases in animals, Energy metabolism, Antigens, Immunoblotting, Enzyme-linked immunosorbent assay, Sheep -- Diseases
Journal or Publication Title:	Research in Veterinary Science
Publisher:	Elsevier Ltd
ISSN:	0034-5288
Date:	June 2008
Volume:	Vol.84
Number:	No.3
Number of Pages:	7
Page Range:	pp. 367-373
Identification Number:	10.1016/j.rvsc.2007.06.004
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
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URI:	http://wrap.warwick.ac.uk/id/eprint/30052

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