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Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus
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Whitworth, David E., Millard, Andrew D., Hodgson, D. A. and Hawkins, Peter F. (2008) Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus. Proteomics, Vol.8 (No.9). pp. 1839-1842. doi:10.1002/pmic.200700544 ISSN 1615-9853.
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Official URL: http://dx.doi.org/10.1002/pmic.200700544
Abstract
We present a novel dataset assessing the specificity of protein-protein interactions between 69 transmitter and receiver domains from two-component system (TCS)-signalling pathways. TCS require a conserved protein-protein interaction between partner transmitter and receiver domains for signal transduction. The complex prokaryote Myxococcus xanthus possesses an unusually large number of TCS genes, many of which have no obvious interaction partners. Interactions between TCS domains of M. xanthus were assessed using a yeast two-hybrid assay, in which domains were expressed as both bait and prey translational fusions. LacZ production was monitored as an indicator of protein-protein interaction, and the strength of interactions classified as weak, medium or strong. Two-hundred and fifty-five transmitter-receiver domain interactions were observed (46 strong), allowing identification of potential signalling partners for individual M. xanthus TCS proteins. In addition, the dataset provides interesting 'meta' information. For instance, many strong interactions were identified between different transmitter domain pairs (34) and receiver domain pairs (23), suggesting a surprisingly large degree of heterodimerisation of these domains. Proteins in our dataset that exhibited similar 'profiles' of interactions, often shared a similar biological function, suggesting that interaction profiles can provide information on biological function, even considering sets of homologous domains.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Library of Congress Subject Headings (LCSH): | Protein-protein interactions, Proteomics, Myxobacterales, Cellular signal transduction | ||||
| Journal or Publication Title: | Proteomics | ||||
| Publisher: | Wiley - V C H Verlag GmbH & Co. | ||||
| ISSN: | 1615-9853 | ||||
| Official Date: | May 2008 | ||||
| Dates: |
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| Volume: | Vol.8 | ||||
| Number: | No.9 | ||||
| Number of Pages: | 4 | ||||
| Page Range: | pp. 1839-1842 | ||||
| DOI: | 10.1002/pmic.200700544 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
| Grant number: | P16665 (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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