Whitworth, David E., Millard, Andrew D., Hodgson, D. A. (David A.) and Hawkins, Peter F.. (2008) Protein-protein interactions between two-component system transmitter and receiver domains of Myxococcus xanthus. Proteomics, Vol.8 (No.9). pp. 1839-1842. ISSN 1615-9853

Full text not available from this repository.

Abstract

We present a novel dataset assessing the specificity of protein-protein interactions between 69 transmitter and receiver domains from two-component system (TCS)-signalling pathways. TCS require a conserved protein-protein interaction between partner transmitter and receiver domains for signal transduction. The complex prokaryote Myxococcus xanthus possesses an unusually large number of TCS genes, many of which have no obvious interaction partners. Interactions between TCS domains of M. xanthus were assessed using a yeast two-hybrid assay, in which domains were expressed as both bait and prey translational fusions. LacZ production was monitored as an indicator of protein-protein interaction, and the strength of interactions classified as weak, medium or strong. Two-hundred and fifty-five transmitter-receiver domain interactions were observed (46 strong), allowing identification of potential signalling partners for individual M. xanthus TCS proteins. In addition, the dataset provides interesting 'meta' information. For instance, many strong interactions were identified between different transmitter domain pairs (34) and receiver domain pairs (23), suggesting a surprisingly large degree of heterodimerisation of these domains. Proteins in our dataset that exhibited similar 'profiles' of interactions, often shared a similar biological function, suggesting that interaction profiles can provide information on biological function, even considering sets of homologous domains.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Protein-protein interactions, Proteomics, Myxobacterales, Cellular signal transduction
Journal or Publication Title:	Proteomics
Publisher:	Wiley - V C H Verlag GmbH & Co.
ISSN:	1615-9853
Date:	May 2008
Volume:	Vol.8
Number:	No.9
Number of Pages:	4
Page Range:	pp. 1839-1842
Identification Number:	10.1002/pmic.200700544
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	P16665 (BBSRC)
References:	[1] Bijlsma, J. J., Groisman, E. A., Making informed decisions: regulatory interactions between two-component systems. Trends Microbiol. 2003, 11, 359–366 [2] Goldman, B. S., Nierman, W. C., Kaiser, D., Slater, S. C. et al., Evolution of sensory complexity recorded in a myxobacterial genome. Proc. Natl. Acad. Sci. USA. 2006, 103, 15200–15205. [3] Yamamoto, K., Hirao, K., Oshima, T., Aiba, H. et al., Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli. J. Biol. Chem. 2005, 280, 1448–1456. [4] Skerker, J. M., Prasol, M. S., Perchuk, B. S., Biondi, E. G., Laub, M. T., Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis. PLoS Biol. 2005, 3, e334. [5] Cock, P. J. A., Whitworth, D. E., Evolution of gene overlaps: relative reading frame bias in prokaryotic two-component system genes. J. Mol. Evol. 2007, 64, 457–462. [6] Marchler-Bauer, A., Panchenko, A. R., Shoemaker, B. A., Thiessen, P. A. et al., CDD: a database of conserved domain alignments with links to three-dimensional structure. Nucleic Acids Res. 2002, 30, 281–283. [7] Higgs, P. I., Cho, K., Evans, L. S., Whitworth, D. E., Zusman, D. R., Four unusual two-component signal transduction homologs, RedC to RedF, are necessary for timely development in Myxococcus xanthus. J. Bacteriol. 2005, 187, 8191–8195. [8] Whitworth, D. E., Hodgson, D. A., Light-induced carotenogenesis in Myxococcus xanthus: evidence that CarS acts as an anti-repressor of CarA. Mol. Microbiol. 2001, 42, 809–819. [9] Pham, V. D., Shebelut, C.W., Jose, I. R., Hodgson, D. A. et al., The response regulator PhoP4, is required for proper sporulation and development-specific phosphatase activities in Myxococcus xanthus. Microbiology 2006, 152, 1609–1620. [10] Estojak, J., Brent, R., Golemis, E. A., Correlation of twohybrid affinity data with in vitro measurements. Mol. Cell. Biol. 1995, 15, 5820–5829. [11] Eisen, M. B., Spellman, P. T., Brown, P. O., Botstein, D., Cluster analysis and display of genome-wide expression patterns. Proc. Natl. Acad. Sci. USA 1998, 95, 14863–14868. [12] Kimura, Y., Mishima, Y., Nakano, H., Takegawa, K., An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress. J. Bacteriol. 2002, 184, 3578–3585. [13] White, R. A., Szurmant, H., Hoch, J. A., Hwa, T., Features of protein-protein interactions in two-component signaling deduced from genomic libraries. Methods Enzymol. 2007, 422, 75–101. [14] Swanson, R. V., Bourret, R. B., Simon, M. I., Intermolecular complementation of the kinase activity of CheA. Mol. Microbiol. 1993, 8, 435–441.
URI:	http://wrap.warwick.ac.uk/id/eprint/30053

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item