Ahmed, Usman, Dobler, Darin, Larkin, Sarah J., Rabbani, Naila and Thornalley, Paul J. (2008) Reversal of hyperglycemia-induced angiogenesis deficit of human endothelial cells by overexpression of glyoxalase 1 in vitro. In: 9th International Symposium on the Maillard Reaction, Munich, Germany, Sep, 2007. Published in: New York Academy of Sciences. Annals, Vol.1126 pp. 262-264.

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Abstract

Dicarbonyl glycation of RGD and GFOGER sites in type IV collagen has been associated with decreased angiogenesis. In this study, we investigated whether overexpression of glyoxalase 1 to decrease dicarbonyl glycation would prevent the angiogenesis deficit induced by hyperglycemia in vitro. Transfection of human microvascular endothelial cells resulted in a four-fold increase in glyoxalase 1 activity compared with controls. Incubation of human microvascular endothelial cells in model hyperglycemia produced a 32% decrease in formation of tube structures that was prevented by glyoxalase 1 overexpression. We conclude that increased protection against dicarbonyl glycation of endothelial cell protein protects hyperglycemia-induced angiogenesis deficit.

Item Type:	Conference Item (Paper)
Subjects:	Q Science > QP Physiology
Divisions:	Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Glyoxalase, Neovascularization, Endothelial cells, Diabetes, Glycosylation
Series Name:	ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
Journal or Publication Title:	New York Academy of Sciences. Annals
Publisher:	Wiley-Blackwell Publishing, Inc.
ISBN:	978-1-57331-719-1
ISSN:	0077-8923
Editor:	Schleicher, E and Somoza, V and Shieberle, P
Date:	April 2008
Volume:	Vol.1126
Number of Pages:	3
Page Range:	pp. 262-264
Identification Number:	10.1196/annals.1433.035
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Wellcome Trust (London, England), Cancer Research UK
Conference Paper Type:	Paper
Title of Event:	9th International Symposium on the Maillard Reaction
Type of Event:	Conference
Location of Event:	Munich, Germany
Date(s) of Event:	Sep, 2007
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URI:	http://wrap.warwick.ac.uk/id/eprint/30066

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