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Preparation of nucleotide advanced glycation endproducts - Imidazopurinone adducts formed by glycation of deoxyguanosine with glyoxal and methylglyoxal
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Fleming, Thomas, Rabbani, Naila and Thornalley, Paul J. (2008) Preparation of nucleotide advanced glycation endproducts - Imidazopurinone adducts formed by glycation of deoxyguanosine with glyoxal and methylglyoxal. In: 9th International Symposium on the Maillard Reaction , Munich, Germany, Sep 1-5, 2007. Published in: New York Academy of Sciences. Annals, Vol.1126 pp. 280-282.
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Official URL: http://dx.doi.org/10.1196/annals.1433.037
Abstract
An analytical procedure was developed for nucleotide advanced glycation endproducts formed by the reaction of glyoxal and methylglyoxal with deoxyguanosine under physiological conditions. For this, the imidazopurinone derivatives, 3-(2'-deoxyribosyl)-6,7-dihydro-6,7-dihydroxyimidazo[2,3-b]purin-9(8)one (dG-G) and 3-(2'-deoxyribosyl)-6,7-dihydro-6,7-dihydroxy-6-methylimidazo-[2,3-b]purine-9(8)one (dG-MG), were prepared. Authentic standard and stable isotope-substituted standard adducts were prepared and an isotopic dilution analysis assay methodology was developed using liquid chromatography with tandem mass spectrometry and optimized DNA extraction and nuclease digestion procedures. Analysis of dG-G, dG-MG, and the oxidative marker 8-hydroxydeoxyguanosine in the DNA of cultured human cells and mononuclear leukocytes showed that nucleotide advanced glycation endproducts are major markers of DNA damage in human cells.
| Item Type: | Conference Item (Paper) |
|---|---|
| Subjects: | Q Science R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School |
| Series Name: | ANNALS OF THE NEW YORK ACADEMY OF SCIENCES |
| Journal or Publication Title: | New York Academy of Sciences. Annals |
| Publisher: | Wiley-Blackwell Publishing, Inc. |
| ISBN: | 978-1-57331-719-1 |
| ISSN: | 0077-8923 |
| Editor: | Schleicher, E and Somoza, V and Shieberle, P |
| Date: | April 2008 |
| Volume: | Vol.1126 |
| Number of Pages: | 3 |
| Page Range: | pp. 280-282 |
| Identification Number: | 10.1196/annals.1433.037 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Conference Paper Type: | Paper |
| Title of Event: | 9th International Symposium on the Maillard Reaction |
| Type of Event: | Other |
| Location of Event: | Munich, Germany |
| Date(s) of Event: | Sep 1-5, 2007 |
| URI: | http://wrap.warwick.ac.uk/id/eprint/30067 |
Data sourced from Thomson Reuters' Web of Knowledge
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