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Preparation of nucleotide advanced glycation endproducts - Imidazopurinone adducts formed by glycation of deoxyguanosine with glyoxal and methylglyoxal
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Fleming, Thomas, Rabbani, Naila and Thornalley, Paul J. (2008) Preparation of nucleotide advanced glycation endproducts - Imidazopurinone adducts formed by glycation of deoxyguanosine with glyoxal and methylglyoxal. New York Academy of Sciences. Annals, Volume 1126 . pp. 280-282. doi:10.1196/annals.1433.037 ISSN 0077-8923.
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Official URL: http://dx.doi.org/10.1196/annals.1433.037
Abstract
An analytical procedure was developed for nucleotide advanced glycation endproducts formed by the reaction of glyoxal and methylglyoxal with deoxyguanosine under physiological conditions. For this, the imidazopurinone derivatives, 3-(2'-deoxyribosyl)-6,7-dihydro-6,7-dihydroxyimidazo[2,3-b]purin-9(8)one (dG-G) and 3-(2'-deoxyribosyl)-6,7-dihydro-6,7-dihydroxy-6-methylimidazo-[2,3-b]purine-9(8)one (dG-MG), were prepared. Authentic standard and stable isotope-substituted standard adducts were prepared and an isotopic dilution analysis assay methodology was developed using liquid chromatography with tandem mass spectrometry and optimized DNA extraction and nuclease digestion procedures. Analysis of dG-G, dG-MG, and the oxidative marker 8-hydroxydeoxyguanosine in the DNA of cultured human cells and mononuclear leukocytes showed that nucleotide advanced glycation endproducts are major markers of DNA damage in human cells.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QP Physiology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Glycosylation, DNA damage, Glyoxal, DNA | ||||
| Journal or Publication Title: | New York Academy of Sciences. Annals | ||||
| Publisher: | Wiley-Blackwell Publishing, Inc. | ||||
| ISBN: | 978-1-57331-719-1 | ||||
| ISSN: | 0077-8923 | ||||
| Editor: | Schleicher, E and Somoza, V and Shieberle, P | ||||
| Official Date: | April 2008 | ||||
| Dates: |
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| Volume: | Volume 1126 | ||||
| Number of Pages: | 3 | ||||
| Page Range: | pp. 280-282 | ||||
| DOI: | 10.1196/annals.1433.037 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Wellcome Trust (London, England), Cancer Research UK | ||||
| Version or Related Resource: | Presented at: 9th International Symposium on the Maillard Reaction, Munich, Germany, Sep 1-5, 2007 | ||||
| Conference Paper Type: | Paper | ||||
| Type of Event: | Other |
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