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Identifying infections with respiratory syncytial virus by using specific immunoglobulin G (IgG) and IgA enzyme-linked immunosorbent assays with oral-fluid samples

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Okiro, Emelda A., Sande, Charles, Mutunga, Martin, Medley, Graham, Cane, Patricia and Nokes, D. James. (2008) Identifying infections with respiratory syncytial virus by using specific immunoglobulin G (IgG) and IgA enzyme-linked immunosorbent assays with oral-fluid samples. Journal of Clinical Microbiology, Vol.46 (No.5). pp. 1659-1662. ISSN 0095-1137

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Official URL: http://dx.doi.org/10.1128/JCM.02190-07

Abstract

Currently, respiratory syncytial virus (RSV) infection is identified in epidemiological studies by virus antigen or nucleic acid detection in combination with serology. Oral-fluid specimens may provide a noninvasive alternative to blood, and oral fluid is more suitable for sampling outside of the clinic setting. We evaluated an indirect enzyme-linked immunosorbent assay for the detection of RSV-specific immunoglobulin G (IgG) and IgA by using oral-fluid samples collected from individuals with RSV infections confirmed by an immunofluorescent antibody test. For five children sampled repeatedly from birth, antibody profiles in oral fluid quite consistently tracked those in paired sera, and RSV infections were detected by rising titers of antibodies of at least one Ig class. Specific IgG responses were generally more reliable than IgA responses, except in early infancy, where the reverse was sometimes true. For a further five young children from whom oral fluid was collected weekly following RSV infection, boosted antibody responses, frequently of a transient nature, lasting a few weeks, were observed; specific IgG responses were of longer duration and more pronounced than specific IgA responses. Our data show significant promise for the use of oral fluid alone in RSV infection surveillance. The observed rapid dynamics of the antibody responses are informative in defining study sampling intervals.

Item Type:

Journal Article

Subjects:

Q Science > QP Physiology
Q Science > QR Microbiology

Divisions:

Faculty of Science > Life Sciences (2010- )

Library of Congress Subject Headings (LCSH):

Respiratory syncytial virus, Enzyme-linked immunosorbent assay, Immunoglobulin G

Journal or Publication Title:

Journal of Clinical Microbiology

Publisher:

American Society of Microbiology

ISSN:

0095-1137

Official Date:

May 2008

Dates:

Date	Event
May 2008	Published

Volume:

Vol.46

Number:

No.5

Number of Pages:

Page Range:

pp. 1659-1662

Identification Number:

10.1128/JCM.02190-07

Status:

Peer Reviewed

Publication Status:

Published

Access rights to Published version:

Restricted or Subscription Access

Funder:

Wellcome Trust (London, England)

Grant number:

061584 (WT), 076278 (WT)

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