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Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography fourier transform ion cyclotron mass spectrometry combined with 18O-labeling
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Wang, Fuyi, Weidt, Stefan, Xu, Jingjing, Mackay, C. Logan, Langridge-Smith, Pat R. R. and Sadler, P. J. (2008) Identification of clusters from reactions of ruthenium arene anticancer complex with glutathione using nanoscale liquid chromatography fourier transform ion cyclotron mass spectrometry combined with 18O-labeling. Journal of The American Society for Mass Spectrometry, Vol.19 (No.4). pp. 544-549. doi:10.1016/j.jasms.2007.12.002 ISSN 1044-0305.
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Official URL: http://dx.doi.org/10.1016/j.jasms.2007.12.002
Abstract
Reactions of the anticancer complex [(eta(6) -bip)Ru(en)Cl](+) (where bip is biphenyl and en is ethylenediamine) with the tripeptide glutathione (gamma-L-GIU-L-Cys-Gly; GSH), the abundant intracellular thiol, in aqueous solution give rise to two ruthenium cluster complexes, which could not be identified by electrospray mass spectrometry (ESI-MS) using a quadrupole mass analyzer. Here we use Fourier transform ion cyclotron mass spectrometry (nanoLC-FT-TCR MS) to identify the clusters separated by nanoscale liquid chromatography as the tetranuclear complex [ {eta(6) -bip)Ru(GSO(2))}(4)](2-) (2) and dinuclear complex [{eta(6) -bip)Ru(GSO(2))(2)}(2)](8-) (3) containing glutathione sulfinate (GSO(2)) ligands. Use of (OH2)-O-18 showed that oxygen from water can readily be incorporated into the oxidized glutathione ligands. These data illustrate the power of high-resolution MS for identifying highly charged multinuclear complexes and elucidating novel reaction pathways for metallodrugs, including ligand-based redox reactions.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QC Physics Q Science > QD Chemistry R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Ruthenium compounds, Antineoplastic agents, Mass spectrometry, Fourier transform spectroscopy, Liquid chromatography, Nanochemistry, Glutathione | ||||
| Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||
| Publisher: | Springer New York LLC | ||||
| ISSN: | 1044-0305 | ||||
| Official Date: | April 2008 | ||||
| Dates: |
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| Volume: | Vol.19 | ||||
| Number: | No.4 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 544-549 | ||||
| DOI: | 10.1016/j.jasms.2007.12.002 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Funder: | Research Councils UK (RCUK), Zhongguo ke xue yuan [Chinese Academy of Sciences], Oncosense Ltd. |
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