Kirilenko, Pavel, Weierud, Frida K., Zorn, Aaron M. and Woodland, Hugh R.. (2008) The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. Differentiation, Volume 76 (Number 4). pp. 392-403. ISSN 0301-4681

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Abstract

A microarray analysis of vegetal pole sequences in the egg and early Xenopus laevis embryo identified Unigene Xl.14891 as a vegetally localized RNA. Analysis of the Xenopus tropicalis genome showed this Unigene to be localized near the 3' end of the Grip2 (glutamate receptor interacting protein 2) transcription unit. RACE showed that the Unigene represented the 3' UTR of Grip2 mRNA. Grip2 mRNA is present in the mitochondrial cloud of late pre-vitellogenic oocytes and then in the germplasm through oogenesis and early development until tailbud tadpole stages. Interference with Grip2 mRNA translation using two antisense morpholino oligos (MOs) impairs primordial germ cell (PGC) migration to the germinal ridges. Both MOs also inhibit swimming movements of the tailbud tadpole, known to involve glutamate receptors. We conclude that Grip2 has several functions in the embryo, including enabling efficient PGC migration.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Xenopus, Germ cells, Germplasm resources, Cell migration, Messenger RNA
Journal or Publication Title:	Differentiation
Publisher:	Elsevier Ltd.
ISSN:	0301-4681
Date:	April 2008
Volume:	Volume 76
Number:	Number 4
Number of Pages:	12
Page Range:	pp. 392-403
Identification Number:	10.1111/j.1432-0436.2007.00229.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Wellcome Trust (London, England), National Institutes of Health (U.S.) (NIH)
Grant number:	HD42572 (NIH)
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URI:	http://wrap.warwick.ac.uk/id/eprint/30272

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