Cakir, Cahid and Tör, Mahmut. (2010) Factors influencing Barley Stripe Mosaic Virus-mediated gene silencing in wheat. Physiological and Molecular Plant Pathology, Vol.74 (No.3-4). pp. 246-253. ISSN 0885-5765

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Abstract

Virus induced gene silencing (VIGS) is a technology that has been used primarily to target the virus genome in infected plants. However, if the virus genome carries inserts derived from the host plant, the system could be employed to target the mRNAs corresponding to the host gene. Barley Stripe Mosaic Virus (BSMV), a disease of various cereals including barley and wheat, has been one of the successfully used viral RNA silencing tools in monocotyledonous plants for the last decade. In this study, we investigated several factors that play a significant role in VIGS. We tested the efficiency of silencing two genes simultaneously using the BSMV-induced gene silencing (IGS) system in detail. We found that two genes could be silenced simultaneously using BSMV-IGS. However, the silencing efficiency was found to be influenced by several factors including stability of the insert, temperature, and the accumulation of small viral RNAs from BSMV. The effect of these factors on VIGS system has been discussed.

Item Type:	Journal Article
Alternative Title:	Virus induced gene silencing in wheat
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Barley stripe mosaic virus -- Research, Wheat -- Diseases and pests, Plant gene silencing, RNA viruses
Journal or Publication Title:	Physiological and Molecular Plant Pathology
Publisher:	Elsevier
ISSN:	0885-5765
Date:	May 2010
Volume:	Vol.74
Number:	No.3-4
Page Range:	pp. 246-253
Identification Number:	10.1016/j.pmpp.2010.04.001
Status:	Peer Reviewed
Access rights to Published version:	Open Access
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URI:	http://wrap.warwick.ac.uk/id/eprint/3029

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