Nuclear factor-kappa B induction by visfatin in human vascular endothelial cells : its role in MMP-2/9 production and activation
Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2008) Nuclear factor-kappa B induction by visfatin in human vascular endothelial cells : its role in MMP-2/9 production and activation. Diabetes Care, Vol.31 (No.4). pp. 758-760. ISSN 0149-5992Full text not available from this repository.
Official URL: http://dx.doi.org/10.2337/dc07-1544
OBJECTIVE - Visfatin is elevated in obesity and type 2 diabetes and is thought to be an inflammatory mediator within atherosclerotic lesions and to induce gelatinase activity. We ;investigated the activation of nuclear factor-kappa B (NF-kappa B), a well-known proinflammatory transcription factor, by visfatin in endothelial cells.
RESEARCH DESIGN AND METHODS - Human endothelial cells were transfected with pNF-kappa B-Luc plasmid. Using quantitative PCR, Western blot analysis, and gelatin zymography, we studied NF-kappa B signaling in gelatinase-mediated vascular inflammation by visfatin using the NF-kappa B inhibitor BAY 11-7085.
RESULTS - Visfatin significantly increased NF-kappa B transcriptional activity (P < 0.001). We also found a significant inhibition of tumor necrosis factor-alpha JN F-alpha) -induced NF-kappa B activityby visfatin (P < 0.001). Furthermore, the NF-kappa B inhibitor significantly negated visfatin-induced matrix metalloprotemase (MMP)-2/9 mRNA expression, protein levels, and gelatinolytic activity (P < 0.001).
CONCLUSIONS - Visfatin-induced NF-kappa B signaling in human endothelial cells affects the activation of gelatinases MMP-2 and -9, suggesting an important role of visfatin in the pathogenesis of vascular inflammation in obesity and type 2 diabetes.
|Item Type:||Journal Article|
|Subjects:||Q Science > QP Physiology
R Medicine > RC Internal medicine
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016)
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Vascular endothelium, NF-kappa B (DNA-binding protein), Cardiovascular system -- Diseases|
|Journal or Publication Title:||Diabetes Care|
|Publisher:||American Diabetes Association|
|Official Date:||April 2008|
|Number of Pages:||3|
|Page Range:||pp. 758-760|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||General Charities of the City of Coventry|
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