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Nuclear factor-kappa B induction by visfatin in human vascular endothelial cells : its role in MMP-2/9 production and activation
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Adya, Raghu, Tan, Bee K., Chen, Jing and Randeva, Harpal S.. (2008) Nuclear factor-kappa B induction by visfatin in human vascular endothelial cells : its role in MMP-2/9 production and activation. Diabetes Care, Vol.31 (No.4). pp. 758-760. ISSN 0149-5992
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Official URL: http://dx.doi.org/10.2337/dc07-1544
Abstract
OBJECTIVE - Visfatin is elevated in obesity and type 2 diabetes and is thought to be an inflammatory mediator within atherosclerotic lesions and to induce gelatinase activity. We ;investigated the activation of nuclear factor-kappa B (NF-kappa B), a well-known proinflammatory transcription factor, by visfatin in endothelial cells. RESEARCH DESIGN AND METHODS - Human endothelial cells were transfected with pNF-kappa B-Luc plasmid. Using quantitative PCR, Western blot analysis, and gelatin zymography, we studied NF-kappa B signaling in gelatinase-mediated vascular inflammation by visfatin using the NF-kappa B inhibitor BAY 11-7085. RESULTS - Visfatin significantly increased NF-kappa B transcriptional activity (P < 0.001). We also found a significant inhibition of tumor necrosis factor-alpha JN F-alpha) -induced NF-kappa B activityby visfatin (P < 0.001). Furthermore, the NF-kappa B inhibitor significantly negated visfatin-induced matrix metalloprotemase (MMP)-2/9 mRNA expression, protein levels, and gelatinolytic activity (P < 0.001). CONCLUSIONS - Visfatin-induced NF-kappa B signaling in human endothelial cells affects the activation of gelatinases MMP-2 and -9, suggesting an important role of visfatin in the pathogenesis of vascular inflammation in obesity and type 2 diabetes.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
| Divisions: | Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Vascular endothelium, NF-kappa B (DNA-binding protein), Cardiovascular system -- Diseases |
| Journal or Publication Title: | Diabetes Care |
| Publisher: | American Diabetes Association |
| ISSN: | 0149-5992 |
| Date: | April 2008 |
| Volume: | Vol.31 |
| Number: | No.4 |
| Number of Pages: | 3 |
| Page Range: | pp. 758-760 |
| Identification Number: | 10.2337/dc07-1544 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | General Charities of the City of Coventry |
| URI: | http://wrap.warwick.ac.uk/id/eprint/30301 |
Data sourced from Thomson Reuters' Web of Knowledge
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