Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome : ex vivo and in vivo regulation of omentin-1 by insulin and glucose
Tan, Bee K., Adya, Raghu, Farhatullah, S., Lewandowski, Kris C., O'Hare, J. Paul, Lehnert, Hendrik and Randeva, Harpal S.. (2008) Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome : ex vivo and in vivo regulation of omentin-1 by insulin and glucose. Diabetes, Vol.57 (No.4). pp. 801-808. ISSN 0012-1797Full text not available from this repository.
Official URL: http://dx.doi.org/10.2337/db07-0990
OBJECTIVE - Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that plasma omentin-1 levels decrease with obesity. Currently, no data exist on the relative expression and regulation of omentin-1 in adipose tissue of women with PCOS. The objective of this study was to assess mRNA and protein levels of omentin-1, including circulating omentin-1, in omental adipose tissue of women with PCOS and matched control subjects. Ex vivo and in vivo regulation of adipose tissue omentin-1 was also studied.
RESEARCH DESIGN AND METHODS - Real-time RT-PCR and Western blotting were used to assess mRNA and protein expression of omentin-1. Plasma omentin-1 was measured by enzyme-linked immunosorbent assay. The effects Of D-glucose, insulin, and gonadal and adrenal steroids on adipose tissue omentin-1 were analyzed ex vivo. The in vivo effects of insulin (hyperinsulinemia) on omentin-1 levels were also assessed by a prolonged insulin-glucose infusion.
RESULTS - In addition to decreased plasma omentin-1 levels in women with PCOS (P < 0.05), compared with control subjects, there was significantly lower levels of omentin-1 mRNA (P < 0.01) and protein (P < 0.05) in omental adipose tissue of women with PCOS (P < 0.01). Furthermore, in omental adipose tissue explants, insulin and glucose significantly dose-dependently decreased omentin-1 mRNA expression, protein levels, and secretion into conditioned media (P < 0.05, P < 0.01). Also, hyperinsulinemic induction in healthy subjects significantly reduced plasma omentin-1 levels (P < 0.01).
CONCLUSIONS - Our novel findings reveal that omentin-1 is downregulated by insulin and glucose. These may, in part, explain the decreased omentin-1 levels observed in our overweight women with PCOS.
|Item Type:||Journal Article|
|Subjects:||Q Science > QP Physiology
R Medicine > RC Internal medicine
R Medicine > RG Gynecology and obstetrics
|Divisions:||Faculty of Medicine > Warwick Medical School > Translational & Systems Medicine > Metabolic and Vascular Health
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Polycystic ovary syndrome -- Research, Obesity, Insulin resistance, Adipose tissues|
|Journal or Publication Title:||Diabetes|
|Publisher:||American Diabetes Association|
|Official Date:||April 2008|
|Number of Pages:||8|
|Page Range:||pp. 801-808|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||General Charities of the City of Coventry|
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