Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts
Bowles, Emma J., Tecirlioglu, R. Tayfur, French, Andrew J., Holland, Michael K. and St. John, Justin C.. (2008) Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts. Stem Cells, Vol.26 (No.3). pp. 775-782. ISSN 1066-5099Full text not available from this repository.
Official URL: http://dx.doi.org/10.1634/stemcells.2007-0747
Following fertilization, mitochondrial DNA is inherited from the oocyte and transmitted homoplasmically. However, following nuclear transfer, mitochondrial DNA can be transmitted from both the donor cell and recipient oocyte, resulting in a state of heteroplasmy. To determine whether the genetic diversity between donor cell and recipient cytoplast mitochondrial DNA influences development, we generated bovine embryos by fusing a donor cell to one or more enucleated cytoplasts. Analysis of mitochondrial DNA from embryos, fetal tissues, and blood samples from offspring revealed that early preimplantation embryos from two or three cytoplasts had significantly more mitochondrial DNA variants than fetal tissues. Phylogenic analysis of embryos generated using single cytoplasts divided the mitochondrial DNA sequence variants into three separate groups with various amounts of genetic divergence from the donor cell line. In heteroplasmic tissue and blood samples, the predominant mitochondrial DNA population was significantly more divergent from the donor cell than the less frequent allele. Furthermore, analysis of the mitochondrially encoded cytochrome B gene showed that two heteroplasmic alleles encoded for different amino acids, and the ratios of mitochondrial DNA/mRNA for each allele differed significantly between tissues. The degree of evolutionary distance between the donor cell and the cytoplast and the variability in heteroplasmy between tissues may have an impact on more divergent intergeneric nuclear transfer and the use of this approach for the generation of embryonic stem cells.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH426 Genetics
Q Science > QP Physiology
|Divisions:||Faculty of Medicine > Warwick Medical School|
|Library of Congress Subject Headings (LCSH):||Mitochondrial DNA, Cloning, Genetic transformation, Genetic transcription, Mitochondrial pathology, DNA replication, Somatic hybrids|
|Journal or Publication Title:||Stem Cells|
|Publisher:||AlphaMed Press, Inc.|
|Number of Pages:||8|
|Page Range:||pp. 775-782|
|Access rights to Published version:||Restricted or Subscription Access|
1 Anderson S, Bankier AT, Barrell BG et al. Sequence and organization of
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